相关实验视频

Updated: Mar 6, 2026

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Published on: June 17, 2015

在暴露于tribuyltin的Tritia mutabilis胚胎中进行miRNome分析:对发育毒性的洞察

Paolo Cocci¹, Gilberto Mosconi¹, Francesco Alessandro Palermo¹

¹School of Biosciences and Veterinary Medicine, University of Camerino, Italy.

Marine pollution bulletin

|March 4, 2026

概括

三乙烯 (TBT) 污染通过改变微RNA (miRNA) 表达方式来破坏海的胚胎发育. 这些变化会影响至关重要的细胞过程,表明miRNAs.

科学领域:

海洋生物学海洋生物学
环境毒理学环境毒理学
分子生物学分子生物学

背景情况:

三甲 (TBT) 是一种持久性海洋污染物,已知会伤害海洋无脊椎动物.
对于TBT影响早期胚胎发育的分子机制尚不清楚.
微RNA (miRNA) 在发育过程中对基因表达的调节起着至关重要的作用.

研究的目的:

调查环境相关的TBT度对海洋类Tritia mutabilis胚胎发生过程中的miRNA表达特征的影响.
为了识别由TBT暴露调节的特定miRNAs.
探索受TBT诱导的miRNA变化影响的生物途径.

主要方法:

暴露Tritia mutabilis胚胎在低 (10-12M) 和高 (10-10M) 的TBT度下,持续10天.
高通量测序用于分析差异性miRNA表达特征.
生物信息路径分析以确定受影响的生物过程.

主要成果:

针对TBT暴露,观察到多个miRNAs的显著调节.
11个miRNA被低度和高度的TBT连续改变.
特定的miRNAs (miR-486-5p,miR-183-5p) 对低TBT作出反应,而其他miR-263b,miR-184,miR-100-5p则对高TBT具有特异性.
受影响的途径包括神经系统发育,细胞增殖,信号通路 (Ras,cAMP,) 和细胞粘附.

结论:

暴露于TBT会改变海洋类胚胎中的miRNA表达,导致发育异常.
通过miRNA介导的基因调节,TBT破坏了关键的细胞功能和通信网络.
miRNAs显示出作为评估TBT污染及其在海洋环境中的发展影响的生物标志物的潜力.

关键词:

内分泌干扰物干扰内分泌干扰物三乙是一种.这就是Tritia mutabilis.发展性毒性发展性毒性这是一个小RNARNA.

更多相关视频

Author Spotlight: High-Throughput Toxicity Screening Using Zebrafish Embryo Startle Response Assay

Author Spotlight: High-Throughput Toxicity Screening Using Zebrafish Embryo Startle Response Assay

Published on: January 12, 2024

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

Published on: April 28, 2017

相关概念视频

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

Teratogenicity

Teratogenicity

The ability of a drug to produce structural deformations and functional abnormalities in the developing embryo or the fetus is called teratogenicity, and the drug producing this effect is known as a teratogen. Teratogenic effects include stillbirth, miscarriage, intrauterine growth restriction, and neurocognitive delay. A teratogen may affect the embryo at different stages of development, which is important in determining the type and extent of the damage. During blastocyst formation, the early...