保存的长非编码RNA LTCONS12135 积极调节 teleost 中的先天免疫力
Yamin Cao1, Xiaoqiong He1, Weiwei Zheng1
1Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
概括
一种新型的长非编码RNA (lncRNA),LTCONS12135,被发现在 miiuy croaker 中,并被证明可以增强先天免疫力. 这种IncRNA增强了水生脊椎动物的抗菌和抗病毒防御.
科学领域:
- * 免疫学 免疫学
- * 分子生物学 * 分子生物学
- * 水生脊椎动物研究
背景情况:
- *长非编码RNAs (lncRNAs) 是已知的哺乳动物先天免疫的调节者.
- * 它们在水生动物免疫反应,特别是抗微生物和抗病毒防御中的作用仍未得到充分研究.
研究的目的:
- * 确定和描述涉及水生动物先天免疫的新型 lncRNA.
- * 为了研究新发现的 lncRNA,LTCONS12135 在 miiuy croaker (Miichthys miiuy) 的免疫反应中的作用.
主要方法:
- * 在M. miiuy细胞 (MSpC) 中识别LTCONS12135.
- *在用脂多糖 (LPS) 和多酸 (poly:polycytidylic acid) 刺激后对LTCONS12135表达的分析.
- *使用斑马鱼 (Danio rerio) 感染Vibrio anguillarum和膀性口腔炎病毒 (VSV) 的模型进行功能验证.
主要成果:
- *LTCONS12135表达在LPS和多I:C刺激后在MSpC显著上调.
- * 在斑马鱼模型中,LTCONS12135增强了促炎性细胞因子和抗病毒基因转录.
- * LTCONS12135有效地抑制了Vibrio anguillarum和膀性口腔炎病毒 (VSV) 的感染和扩散.
结论:
- * LTCONS12135在米乌伊·克罗克的抗菌和抗病毒先天免疫反应中起着至关重要的作用.
- *这些发现突出了远物种中 lncRNAs 保持的免疫调节功能.
- * 非编码RNA在塑造水生脊椎动物的先天免疫系统方面具有重要作用.
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