干扰素诱导的蛋白44-Like在Amphiprion clarkii的功能性特征:表达特征,免疫调节活性和抗病毒功能
Yasara Kavindi Kodagoda1, D C G Rodrigo1, U P E Arachchi1
1Department of Marine Life Sciences & Center for Genomic Selection in Korean Aquaculture, Jeju National University, Jeju 63243, Republic of Korea.
Fish & shellfish immunology
|March 4, 2026
概括
黄尾小丑鱼中的干扰素诱导的44样蛋白 (IFI44L) 对病毒性出血性败血症病毒 (VHSV) 显示出强烈的抗病毒活性. 这种鱼FI44L增强了免疫信号,为水产养殖疾病治疗提供了潜力.
科学领域:
- 免疫学 免疫学 免疫学
- 水生生物学 水生生物学
- 分子生物学分子生物学
背景情况:
- 干扰素诱导的44类蛋白 (IFI44L) 是干扰素刺激的关键基因 (ISG),参与天生的抗病毒免疫力.
- 了解不同物种中的IFI44L功能对于开发广泛的抗病毒策略至关重要.
研究的目的:
- 在黄尾小丑鱼 (Amphiprion clarkii) 中识别和功能性描述IFI44L同类物 (AcIFI44L).
- 研究AcIFI44L对病毒性出血性败血症病毒 (VHSV) 的抗病毒作用和免疫调节机制.
主要方法:
- 对AcIFI44L的基因鉴定和序列分析.
- 在免疫刺激下对AcIFI44L的表达分析.
- 在鱼类细胞系中进行过度表达研究,以评估对VHSV的抗病毒活性.
- 对NF-κB信号通路激活的分析.
主要成果:
- 鉴定了AcIFI44L基因的保存域;转录在免疫组织中丰富,并通过刺激进行上调.
- 过度表达AcIFI44L显著抑制了VHSV复制,并减少了鱼细胞中的病毒载荷.
- AcIFI44L增强了NF-κB信号传递,表明它在调节宿主免疫反应中的作用.
结论:
- 在黄尾小丑鱼中,IFI44L具有显著的物种特异性抗病毒作用.
- AcIFI44L显示出强大的VHSV抑制,突出其作为水产养殖中的病毒性疾病治疗点的潜力.
更多相关视频
10:00High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Published on: March 24, 2015
13.9K
06:50Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
Published on: January 26, 2024
2.7K
相关概念视频
Mechanism of Filopodia Formation
3.3K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
3.3K
Leaky Scanning
5.8K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.8K
Protein Complexes with Interchangeable Parts
3.0K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
3.0K
Lampbrush Chromosomes
8.8K
In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...
8.8K
