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相关实验视频

Updated: Mar 6, 2026

Author Spotlight: Advancing Large-Scale Neural Dynamics Through HD-MEA Technology
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开源的模块化现场可编程门阵列系统用于双光子测距仪,可实现多区域,多深度的神经活动记录和终身成像.

Riichiro Hira1,2, Fumiya Imamura1, Hiroto Imamura1

  • 1Institute of Science Tokyo, Graduate School of Medical and Dental Sciences, Department of Physiology and Cell Biology, Tokyo, Japan.

Neurophotonics
|March 5, 2026
PubMed
概括

我们开发了一个开源的双光子显微镜系统,使用FPGA和CDP模块来提高成像速度并添加光寿命成像. 该系统允许更快,更深,更广泛的脑成像,有利于神经科学研究和生物医学应用.

关键词:
在FPGA中,FPGA是指FPGA.大脑皮层 大脑皮层四个平面成像系统.终身成像成像技术 终身成像弥苏镜是指一个可视的镜头.多重复杂的多重复杂.这是一个开源的开源软件.两个光子的两个光子.

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相关实验视频

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科学领域:

  • 神经科学是一个神经科学.
  • 生物医学工程 生物医学工程
  • 光学成像技术的成像

背景情况:

  • 大视野 (FOV) 两光子显微镜对于中介尺度神经记录至关重要,但由于扫描时间长,其时间分辨率受到限制.
  • 现有的系统往往缺乏模块化和光寿命成像能力,阻碍了高级神经科学研究的更广泛的可访问性.

研究的目的:

  • 开发和验证一个开源的模块化平台,结合现场可编程门阵列 (FPGA) 和循环延迟路径 (CDP) 模块.
  • 为了实现高吞吐量,多区域,多深度中介尺度两光子成像和大FOV两光子光寿命成像 (2p-FLIM).

主要方法:

  • 建立了一个FPGA系统,以以3.2GS/s的速度将光倍增器信号数字化.
  • 集成了一个CDP模块,用于在四个焦点平面上进行激发的时间复杂化,FPGA处理解复杂化和图像重建.
  • 2p-FLIM是在同一个FPGA+CDP平台上实现的.

主要成果:

  • 该系统同时记录了两侧背皮质的1万多个神经元,深度高达4个.
  • 证明了大FOV 2p-FLIM能力.
  • 所有的硬件和软件都是开源的,并且与现有的双光子显微镜兼容.

结论:

  • 模块化,开源的FPGA + CDP系统显著增加了大FOV两光子成像的吞吐量.
  • 它增加了光寿命对比度,而不需要专门的激光器,扩大了对中等尺度神经测量的访问.
  • 促进了多规模的体内研究和多种生物医学应用.