在患有全身性红血性狼的患者中,氏酸3-酶 δ活性
Elham Sadat Mirfazeli1,2, Shalmalee Kharkar1,2, Michel W P Tsang-A-Sjoe3
1Molecular Cell Biology and Immunology, Amsterdam UMC Location Vrije Universiteit Amsterdam, Amsterdam, Netherlands.
Frontiers in immunology
|March 5, 2026
概括
系统性红斑狼 (SLE) 患者通常不会表现出T细胞氨酸 3-酶三角酶 (PI3Kδ) 活性增加. 然而,SLE患者的一小部分患者表现出显著升高的PI3Kδ活性,这需要进一步研究针对性治疗.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 系统性红斑狼 (SLE) 需要新的生物标志物来改善患者分层和个性化治疗策略.
- 氨基酸3-酶三角酶 (PI3Kδ) 在SLE病变发生过程中发挥作用,使其活性成为研究的潜在目标.
研究的目的:
- 调查一组SLE患者是否在T细胞激活后表现出PI3Kδ活性增加.
- 评估PI3Kδ活动作为SLE生物标志物的潜力.
主要方法:
- 从SLE患者和健康对照中分离出T细胞.
- 细胞被刺激,用高性能液体染色体质谱法测量了酸3,4,5-三酸盐 (PIP3) 和酸4,5-二酸盐 (PIP2) 的水平.
主要成果:
- 一组SLE患者 (n=4) 在刺激后显著增加了PIP3水平,与激活PI3K Delta综合征 (APDS) 患者相比.
- 在一般的SLE队列和健康对照之间没有观察到PI3Kδ活性的总体显著差异.
- 皮普3水平与特定的T细胞子集频率相关 (CD4+,CD8+,EMRA CD4+,EMRA CD8+).
结论:
- 增加PI3Kδ活性不是SLE的一般特征,但存在于不同的患者亚组中.
- 这种PI3Kδ活性较高的子组可能受益于PI3Kδ抑制剂疗法.
- 为治疗开发,建议对这种特定的SLE亚组进行进一步的研究.
更多相关视频
08:07Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
Published on: July 26, 2019
9.0K
11:44A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3
Published on: January 24, 2016
12.6K
相关概念视频
01:42Phosphoinositides and PIPs
10.4K
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
10.4K
01:20The JAK-STAT Signaling Pathway
13.5K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
13.5K
01:11IP3/DAG Signaling Pathway
15.3K
Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
15.3K
01:22PI3K/mTOR/AKT Signaling Pathway
6.0K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
6.0K
01:22Amplifying Signals via Enzymatic Cascade
18.8K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
18.8K