在亚细胞毒性水平的银纳米粒子通过破坏肠道上皮屏障完整性来增加肠道病原体的入侵
Radwa A Hanafy1, Kuppan Gokulan1, Sangeeta Khare1
1Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, AR, Jefferson, United States.
Frontiers in cellular and infection microbiology
|March 5, 2026
概括
银纳米颗粒 (AgNPs) 可以通过损害表皮防御来增加肠道病原体的入侵和持久性. 即使是低度的AgNP也会破坏肠道屏障的完整性,可能会对消费者产品造成健康风险.
科学领域:
- 纳米技术 纳米技术
- 毒理学 毒理学 毒理学
- 微生物学 微生物学
背景情况:
- 银纳米粒子 (AgNPs) 具有抗菌性质,导致它们被纳入食品包装和食补充剂等消费产品中.
- AgNPs对肠道上皮质完整性和对细菌感染的易感性的影响需要进一步调查.
- 以前的研究表明,暴露于AgNP会改变基因表达,细胞因子分泌和肠道微生物群.
研究的目的:
- 为了评估AgNP对肠道屏障宿主-病原体相互作用的影响.
- 确定AgNP对人类肠道细胞中*Salmonella*Heidelberg血清菌的粘附,入侵和细胞内持久性的影响.
- 评估AgNP对表皮屏障透性,基因表达和细胞因子反应的影响.
主要方法:
- 人类肠道上皮细胞 (T84) 经过10nmAgNP的预处理,其度低于细胞毒性.
- 评估了细菌 (*Salmonella* 黑德堡血清菌) 的粘附性,入侵性和细胞内持久性.
- 测量了上皮屏障的透性,基因表达特征和细胞因子分泌.
主要成果:
- AgNP预处理 (10μg/mL) 显著增加了细菌入侵和细胞内持久性,以度依赖的方式.
- AgNP上调调节了与肠道透性和细胞结合相关的基因,这表明修复机制受损.
- 在AgNP暴露后,促炎性细胞因子 (IL-18,TNF-α) 的分泌量增加,而抗炎性细胞因子 (G-CSF,IL-1ra) 减少.
结论:
- 即使是低度的AgNP暴露,也会破坏肠道屏障的完整性,促进病原体的入侵和持久性.
- 这些发现突出了与从消费品中慢性摄入AgNP相关的潜在健康风险.
- 需要重新评估关于胃肠道健康和宿主-病原体相互作用的AgNP安全性.
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