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波洛克萨默稀释作为根据需求的替代品,以阿加稀释为基础的抗微生物敏感性测试

Matthew T J Uy^1,2, Andrea Kirmaier¹, Lindsey M Rudtner¹

¹Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.

Journal of clinical microbiology

|March 5, 2026

概括

一种新的波洛克萨默稀释方法为抗生素敏感性测试提供了传统阿加稀释的实用替代方案. 这种对温度敏感的凝剂可以快速准备试验板,提供准确的结果,用于氏菌阴性病原体的氏菌素敏感性.

科学领域:

临床微生物学临床微生物学
抗微生物敏感性测试试验抗微生物敏感性测试
制药科学制药科学

背景情况:

阿格拉稀释是抗菌素敏感性测试的参考方法,但对于临床实验室来说是劳动密集的.
目前用于测试菌素敏感性的方法,特别是对于大肠杆菌尿路感染,缺乏参考质量的最小抑制度 (MIC) 数据.
需要实用,按需的敏感性测试方法,可以在临床环境中实施.

研究的目的:

评估波洛克萨默稀释作为一种快速的,按需替代传统亚格稀释的抗菌敏感性测试.
评估波洛克萨默稀释剂对包括*Escherichia coli*,*Enterobacterales*和*Pseudomonas aeruginosa*在内的グラム阴性病原体的菌素敏感性测试的性能.
确定Poloxamer稀释在临床实验室环境中的实际用途.

主要方法:

波洛克萨默407是一种温度敏感的凝剂,用于替代Bacto agar,用于准备敏感性测试板.
波洛克萨默溶液在冷却温度下保持液态,在加热后固化,使得可以按需准备板块.
用波洛克萨默稀释和参考甲稀释方法对斯福米辛进行了针对各种グラム阴性病原体的敏感性测试.

主要成果:

波洛克萨默稀释表现出与*Escherichia coli* (100% CA,87% EA) 的稀释具有优异的分类一致性 (CA) 和基本一致性 (EA).
对其他*Enterobacterales* (82%) 观察到良好的CA和EA,对*Pseudomonas aeruginosa* (60%) 观察到较低的CA,这是由于MIC接近断点.
波洛克萨默稀释显著减少了 * Proteus * spp. 的跳过稀释,并抑制了 * Proteus * spp. 的蜂群,没有非常大的错误,并且观察到很少的重大错误.

结论:

波洛克萨默稀释是一种实用和有效的按需替代阿加稀释,用于对格拉姆阴性病原体的菌素敏感性测试.
这种方法在实践中提供了与稀释相同的性能,并且可以在没有特殊设备的情况下在临床实验室中实施.
波洛克萨默稀释的实用性值得进一步探索其他抗菌素敏感性测试应用.

关键词:

进入细菌体中的细菌.穆勒希顿中等的中等普鲁罗尼克F-127是一架飞机.波洛克萨默 407 的 407 号伪omonas空气菌伪omonas空气菌阿加尔稀释剂的稀释方法对抗微生物的敏感性测试.临床微生物学实验室临床微生物学实验室这种药物是斯福米辛 (phosfomycin).格拉姆阴性细菌是一种细菌.

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