在番茄和其他作物中,通过细胞大小调节器 (CSR) 的类似物调节水果重量
Qian Feng1, Lara Pereira2, Manoj Sapkota1,3
1Institute of Plant Breeding, Genetics and Genomics, University of Georgia, Athens, GA, USA.
概括
研究人员将CSR-like1确定为一种新型基因,该基因通过增加周骨细胞大小来调节番茄水果的体重. 这一发现与fw6.2 QTL相关,表明其在其他作物中保留了功能,影响了水果化.
科学领域:
- 植物遗传学 植物遗传学
- 农作物化 农作物化
- 定量特征位置 (QTL) 分析分析.
背景情况:
- 水果重量是影响作物化和选择的关键定量特征.
- 在番茄 (Solanum lycopersicum) 中发现了大量的果实重量QTL,但很少有基因被克隆.
- 了解水果重量的遗传基础对于提高作物质量至关重要.
研究的目的:
- 确定控制番茄果实体重的新型遗传位置.
- 克隆和鉴定 fw6.2 QTL.基因的基因.
- 调查已识别的基因的进化历史和潜在的保存.
主要方法:
- 基因组广泛关联研究 (GWAS) 和各种番茄面板中的链接映射.
- 在染色体6 (fw6.1和fw6.2) 上精细地映射QTLs.
- 使用近同位基因线 (NIL) 和转基因方法识别和功能验证候选基因.
- 在胡中进行哈普洛型分析和ortolog识别.
主要成果:
- 两个水果重量QTLs,fw6.1和fw6.2,在染色体6上进行了映射.
- CSR-like1被确定为fw6.2 QTL的候选基因.
- 具有CSR-like1-Derived等位基的NILs表现出更重的果实和更大的周周细胞.
- 转基因下调的CSR-like1减少了水果的重量和周骨细胞的大小.
- 哈普洛型分析表明,在番茄化过程中选择了CSR-like1-Derived等位基因.
- 在CSR类1监管区域的SNP与水果重量和细胞大小有关.
- 胡中的CSR-like1 ortolog也与水果重量有关.
结论:
- CSR-like1是一种新型基因,通过控制骨细胞大小来调节番茄水果的体重.
- 已识别的fw6.2 QTL是由CSR-like1支的,其中一个选择的等位基因有助于增加水果重量.
- 在水果重量中,CSR-like1的功能似乎在Solanaceae作物中保持不变,包括胡.
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