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UV–Vis Spectrometers01:14

UV–Vis Spectrometers

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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
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Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
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便携式色度吸收读数器通过时间分辨率传感扩展动态范围.

S Moreno1, O Caravaca-Müller2, X García-Fernández3

  • 1Department of Electronic and Biomedical Engineering, Faculty of Physics, Universitat de Barcelona (UB), Barcelona, Spain; Institute for Nanoscience and Nanotechnology (IN2UB), Universitat de Barcelona (UB), 08028, Barcelona, Spain.

Talanta
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概括

本研究介绍了一种使用单光子雪崩二极管 (SPAD) 阵列进行时间分辨率光子计数的便携式阅读器. 这种先进的吸收率读取器扩展了动态范围,并改善了生物分析方法的量化,增强了临床诊断.

关键词:
卷积神经网络 (CNN) 是一种神经网络.高动态范围的高动态范围.磁性免疫测试是一种磁性免疫测试.一个便携式的吸收率读取器.一个光子雪崩二极管 (SPAD)时间分辨率感应传感器

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科学领域:

  • 生物分析化学 生物分析化学
  • 光子学 是一个光子学.
  • 医学诊断 医学诊断 医学诊断

背景情况:

  • 颜值测量生物分析方法通常在高分析度下和,需要连续稀释.
  • 传统的吸收率读数器对具有不同分析剂水平的样品具有动态范围的限制.

研究的目的:

  • 开发一款便携式吸收率读数器,使用时间解析光子计数扩展动态范围.
  • 为了克服生物分析试验中传统读数器的和限制.

主要方法:

  • 使用单光子雪崩二极管 (SPAD) 阵列进行像素内时间分辨率光子计数.
  • 采用基于生命周期的测量原理,从光子到达衰变常数 (τ) 导出吸收率.
  • 将读器应用于B型流感核蛋白磁粒子免疫试验.

主要成果:

  • 基于SPAD的阅读器表现出高达64 ng/mL的单调响应,明显超过商业光度仪的12.5 ng/mL和度.
  • 实现了低检测值 (0.0034 ng/mL) 和定量值 (0.0949 ng/mL),变化系数<10%.
  • 一个卷积神经网络从时间解析的光子数据中准确推断出分析物度 (R2 = 0.986).

结论:

  • 基于SPAD的阅读器扩展了可用的吸收范围,并保持了灵敏度,性能优于商业设备.
  • 时间解析光子计数方法允许物理建模和数据驱动推理进行准确的量化.
  • 这项技术为高性能,便携式诊断解决方案提供了一条途径.