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一种二基化酶EuPPPDE8,通过与Eucommia ulmoides中的EuFPS1和EuMGRN1相互作用,促进的生物合成

Huaijuan Jiao¹, Dan Zhao², Xi Chen³

¹The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/College of Tea Sciences, Guizhou University, Guiyang, 550025, China; Plant Conservation & Breeding Technology Center, Guizhou Academy of Agricultural Sciences, Guiyang, 550006, China.

International journal of biological macromolecules

|March 5, 2026

概括

含有酶8 (EuPPPDE8) 的Eucommia ulmoides peptidase域的基因促进了的生物合成. 过度表达增加了51.51%的含量,而沉默减少了它,突出了它在Eucommia生产中的作用.

科学领域:

植物分子生物学植物分子生物学
生物化学生物化学
遗传学是一个遗传学.

背景情况:

植物酸酶域含酶 (PPPDE) 基因家族对植物生长,发育,应激反应和信号传导至关重要.
欧康米亚乌尔莫伊德 farnesyl pyrophosphate synthase 1 (EuFPS1) 是生物合成中的一个关键酶,其相互作用的蛋白质具有重大意义.

研究的目的:

系统地识别和描述Eucommia ulmoides中的PPPDE家族成员.
为了研究Eucommia ulmoides皮酶域含酶8 (EuPPPDE8) 在Eucommia ulmoides生物合成中的作用.
探索EuPPPDE8与EuFPS1和其他蛋白质的相互作用.

主要方法:

对PPPDE家族成员进行全基因组识别和遗传学分析.
基因克隆,酵母双混合 (Y2H) 和双分子光补充 (BiFC) 试验以确认蛋白质相互作用.
基因过度表达和沉默实验,以评估生物合成中的功能性作用.
在低温压力下对基因表达的分析.

主要成果:

在Eucommia ulmoides中确定了9个PPPDE家族成员,在生产植物中显示出高同质性.
EuPPPDE8与EuFPS1和Eucommia ulmoides mahogunin戒指1 (EuMGRN1) 相互作用. 这两种类型的蛋白质都存在相互作用.
EuPPPDE8的表达因低温而下调,与含量降低相关.
过度表达EuPPPDE8显著增加了51.51%的Eucommia含量,而静音降低了它.

结论:

EuPPPDE8是一种二基化酶,在促进Eucommia ulmoides生物合成方面发挥着至关重要的作用.
EuPPPDE8可能通过其deubiquitinating活性对抗EuMGRN1-介导的蛋白质分解.
这项研究提供了基础数据,以了解生物合成中的二化调节.

关键词:

消灭城市的消灭.欧巴巴PDE8 在线阅读尤科米亚乌尔莫伊德斯 (Eucommia ulmoides) 是一种植物.在PPPDE基因家族中,酵母两个混合 (Y2H) /双分子光补充 (BiFC)

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