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相关概念视频

Structural Classification of Joints01:20

Structural Classification of Joints

8.0K
Joints, also known as articulations, are classified based on their structural characteristics, i.e., based on whether the articulating surfaces of the adjacent bones are directly connected by fibrous connective tissue or cartilage, or whether the articulating surfaces contact each other within a fluid-filled joint cavity. These differences serve to divide the joints of the body into three structural classifications.
A fibrous joint is where the adjacent bones are united by fibrous connective...
8.0K
Functional Classification of Joints01:09

Functional Classification of Joints

8.5K
Functional Classification of Joints
The functional classification of joints is determined by the amount of mobility between the adjacent bones. Joints are functionally classified as a synarthrosis or immobile joint, an amphiarthrosis or slightly moveable joint, or as a diarthrosis, a freely moveable joint. Fibrous and cartilaginous joints can be functionally classified as either synarthroses  or amphiarthroses, whereas all synovial joints are classified as diarthroses.
Synarthrosis
An...
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Genome Annotation and Assembly03:36

Genome Annotation and Assembly

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
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Ligand Binding and Linkage00:49

Ligand Binding and Linkage

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Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
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相关实验视频

Updated: Mar 7, 2026

Author Spotlight: 3D Movement Assessment of Maxillary Posterior Teeth in Clear Aligner Treatment
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Author Spotlight: 3D Movement Assessment of Maxillary Posterior Teeth in Clear Aligner Treatment

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结构对齐器在功能域注释中的比较性能

Poorya Mirzavand Borujeni1, Reza Salavati2

  • 1Institute of Parasitology, McGill University, Canada.

Journal of structural biology
|March 5, 2026
PubMed
概括

使用AlphaFold模型的基于结构的蛋白质域注释显示出希望,Reseek在灵敏度方面表现出色. 然而,像HMMER这样的序列配置文件方法对于家族级别的注释仍然优越,特别是当包含配置文件信息时.

科学领域:

  • 计算生物学 计算生物学
  • 结构生物信息学 结构生物信息学
  • 蛋白质信息学 蛋白质信息学

背景情况:

  • 准确的蛋白质域注释对于理解蛋白质功能至关重要.
  • 像Pfam这样的数据库使用序列衍生签名,但基于结构的方法为远距离相关的蛋白质提供了更高的灵敏度.
  • AlphaFold提供精确的蛋白质结构预测,使新的基于结构的注释方法成为可能.

研究的目的:

  • 系统地评估Pfam域注释的基于结构的与基于序列的方法.
  • 将结构对齐器 (Reseek,Foldseek,TM-align) 与基于序列的方法 (MMseqs,HMMER) 进行比较.
  • 评估来自个人资料的信息对结构注释性能的影响.

主要方法:

  • 三个结构对齐器和两种基于序列的方法的基准测试,使用所有对所有和分裂家族的搜索.
  • 对Pfam多个序列对齐的残留水平对齐精度的评估.
  • 对结构性命中排名的配置信息重定位的调查.

主要成果:

  • 在所有对所有搜索中,Reseek表现出最高的灵敏度 (AUC=0.85),优于其他结构和序列对齐器.
  • 在分裂家族评估中,HMMER仍然优越 (最大F1=0.991),证实了序列配置文件方法的强度.
关键词:
阿尔法折叠是什么意思阿尔法折叠域名注释 域名注释普法姆 (Pfamfam) 是一个家庭.结构调整结构调整

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  • 结构对齐器提供了比对对序列方法更准确的残留水平映射.
  • 基于配置文件的重定位提高了短域名的性能.

    • 结论:

    • 基于结构的方法,特别是Reseek,对蛋白质域注释具有很高的灵敏度,特别是在低序列标识时.
    • 像HMMER这样的序列配置方法仍然是家庭级注释的最佳方法.
    • 将结构信息与序列配置文件结合起来,为增强的域注释提供了一个有希望的方向.