用于膀癌检测的糖蛋白生物标记物
Abida Khan1, Fadiyah Jadid Alanazi2, Saif Ahmed3
1Center For Health Research, Northern Border University, Arar 73213, Saudi Arabia; Department of Pharmaceutical Chemistry, College of Pharmacy, Northern Border University, Rafha, Saudi Arabia.
概括
尿液中的葡萄糖蛋白和葡萄糖因子作为膀癌 (BC) 检测和非肌肉侵入性 (NMIBC) 和肌肉侵入性 (MIBC) 形式之间的差异化的非侵入性生物标志物具有前景. 为了在患者监测和诊断中临床应用,需要进一步验证.
科学领域:
- 生物化学 生物化学
- 在瘤学瘤学.
- 生物标志物发现发现
背景情况:
- 囊镜是侵入性的;尿细胞学对低度膀癌 (BC) 的敏感性较低.
- 瘤相关的糖化变化 (N-和O-糖化,化,化) 是潜在的BC生物标志物.
- 尿液中的葡萄糖蛋白和葡萄糖,特别是在细胞外囊泡 (EVs) 中,正在作为非侵入性诊断指标进行研究.
研究的目的:
- 审查关于膀癌 (BC) 尿液糖蛋白和甘氨酸生物标志物的当前发现.
- 评估它们在区分非肌肉侵入性 (NMIBC) 和肌肉侵入性 (MIBC) 膀癌中的有效性.
- 探索它们在治疗后患者监测中的实用性.
主要方法:
- 用质谱法,基于莱克的测定和向免疫测定研究的文献综述.
- 分析多重尿液糖蛋白面板和EV衍生的N-甘氨酸签名.
- 评估分析前标准化挑战和分析可行性.
主要成果:
- 尿液糖蛋白板显示高的诊断准确度 (AUC 0.82-0.96) 和比细胞学更好的灵敏度低级BC.
- 来自EV的N-甘氨酸签名表明了初步的NMIBC-MIBC歧视 (AUC为0.86-0.88).
- 预分析标准化是临床翻译的关键障碍.
结论:
- 尿液中的葡萄糖蛋白和葡萄糖生物标志物为膀癌 (BC) 检测和分层提供了一个有希望的非侵入性方法.
- 这些生物标志物可以作为囊镜的辅助剂,用于血液分拣和监测.
- 未来的多中心验证,监管评估和健康经济分析对于临床实施至关重要.
相关概念视频
03:31Labeling DNA Probes
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
01:15Western Blotting
Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.