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对含有PFAS和双醇的聚乙烯微塑料混合物的重复暴露会激活具有炎症特征的THP-1巨细胞

Lucas Gaillard¹, Léo Sportes-Milot¹, Kloé Debizet¹

¹INSERM, Health & Functional Exposomics - HealthFex unit, Université Paris Cité, Paris, France.

Environmental pollution (Barking, Essex : 1987)

|March 5, 2026

概括

对微塑料 (MP) 和化学添加剂的重复暴露会在免疫细胞中引发炎症反应. 这项研究揭示了MPs混合物激活巨细胞,增加促炎性细胞因子的释放和改变细胞代谢,突出显示环境污染物的风险.

科学领域:

环境毒理学环境毒理学
细胞生物学细胞生物学
免疫学免疫学免疫学

背景情况:

微塑料 (MPs) 是普遍存在的环境污染物,由于它们的聚合物性质和相关的化学添加剂,如双 (BPs) 和per-和多基物质 (PFAS),它们具有毒性风险.
在MPs和添加剂的联合毒性方面存在有限的体外研究,特别是关于模仿人类污染的反复暴露机制.

研究的目的:

评估聚乙烯微塑料 (PE-MPs) 和添加剂混合物 (BPA,BPS,PFOS,PFOA) 对THP-1巨细胞的明显毒性影响.
研究单次和重复暴露 (超过五天) 对巨细胞反应和潜在分子机制的影响.

主要方法:

在一次性和重复性暴露条件下,THP-1巨体被暴露在PE-MPs (100μg/mL) 和添加剂混合物 (AddMix,BPA,BPS,PFOS,PFOA各10μM) 中.
使用扫描电子和拉曼显微镜证实了PE-MPs的内部化.
分析了细胞反应,包括线粒体ROS产生,甘油性表型,IL-1β分泌和基因表达 (转录组学).

主要成果:

PE-MPs被巨细胞有效地内化.
对PE-MPs和PE-MPs+AddMix的重复暴露显著增加了线粒体ROS产量 (分别为19%和13%),并促进了糖溶性表型.
暴露于PE-MPs导致IL-1β分泌显著增加 (38%),转录组分析揭示了与炎症和脂质代谢相关的基因的增强表达.

结论:

微塑料添加剂混合物,特别是在重复暴露后,被巨细胞识别为强大的激活剂.
这些暴露刺激炎症通路并改变细胞代谢,强调了与复杂的环境污染物混合物相关的潜在健康风险.
需要进一步的研究,以充分阐明这些观察到的炎症反应背后的机制.

关键词:

炎症炎症是一种炎症.巨细胞的两极分化在ROS生产生产中,ROS产量文字转录学 (Transcriptomics) 是一个学科.

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