具有成本效益的植物基媒介用于增强B. amyloliquefaciens CN12的子生产,用于生物肥料应用
Tuan Ngoc Nguyen1, Tu Cam Ly2, Nghi Tran3
1Group of Applied Research in Advanced Materials for Sustainable Development, Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, Vietnam. nguyenngoctuan@tdtu.edu.vn.
Scientific reports
|March 5, 2026
概括
从森林土壤中分离出来的Bacillus amyloliquefaciens菌株CN12,显示出作为生物肥料的潜力. 优化的生长条件产生了高子产量,显著提高了马拉巴尔菜的植物生长指标.
科学领域:
- 农业微生物学 农业微生物学
- 植物科学 植物科学
- 生物技术是生物技术.
背景情况:
- 形成内胞的细菌对土壤健康和植物生长至关重要.
- 生物肥料为化学肥料提供了一个可持续的替代品.
- 识别具有促进植物生长特性的新型细菌菌株对于农业创新至关重要.
研究的目的:
- 为生物肥料应用,从坦丰森林中分离和描述形成内胞的细菌.
- 评估选定细菌菌株的促进植物生长的能力.
- 为一个有前途的候选菌株优化内胞的生产.
主要方法:
- 从森林土壤样本中分离和选形成内胞的细菌.
- 评估促进植物生长的特征,包括英多尔-3-酸的产生, siderophore 分泌和/溶解.
- 使用16S rRNA基因测序识别所选菌株.
- 液体培养基的优化,以最大限度地提高内胞产量.
- 对生物肥料对马拉巴菜生长参数的有效性进行评估.
主要成果:
- 确定了12种具有印醇-3-酸生产的内胞形成细菌.
- 被认定为Bacillus amyloliquefaciens的CN12菌株表现出优越的植物生长促进特征.
- 优化的介质配方 (mung豆芽提取物,糖蜜,尿素,MgSO4) 产生了5.53 × 10^8 CFU/mL的内.
- 用CN12菌株治疗显著增加了马拉巴菜的新鲜重量 (高达77.6克),植物高度 (高达65.8厘米) 和根重量 (高达6.8克).
结论:
- 乙氨基菌菌株CN12是一种强大的促进植物生长的细菌.
- 优化的介质有效地增强了用于生物肥料配方的内胞生产.
- 菌株CN12显示出开发成商业生物肥料产品的巨大潜力.
相关概念视频
Microbes in the Production of Fermented Foods
330
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
330
Bioreactor Controls-III
70
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
70
Production of Organic Acids
111
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
111
Production of Antibiotics
281
Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...
281
Production of Biopesticides
120
Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...
120
Biofuels
108
The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...
108


