识别皮肤暴露于空气污染和皮肤屏障损伤之间的共享诊断基因和潜在机制:一种生物信息学方法
Yueyue Chen1,2, Liping Qu1,2,3, Feifei Wang1,2,3
1Yunnan Botanee Bio-Technology Group Co., Ltd., Kunming, Yunnan, China.
Environmental toxicology
|March 5, 2026
概括
空气污染会通过引起炎症和屏障损伤来损害皮肤. 这项研究确定FOSL1和TKT是空气污染引起的皮肤损伤的关键基因和潜在生物标志物.
科学领域:
- 皮肤病学 皮肤病学
- 环境健康 环境健康
- 基因组学就是基因组学.
背景情况:
- 空气污染显著影响皮肤健康,导致炎症,过早衰老和表皮屏障功能受损.
- 识别分子机制和生物标志物对于理解和减轻这些影响至关重要.
研究的目的:
- 确定与皮肤暴露在空气污染 (PS) 和皮肤屏障损伤 (SD) 相关的基因.
- 发现空气污染引起的皮肤疾病的潜在诊断生物标志物.
主要方法:
- 用于PS和SD的基因表达总体 (GEO) 数据集.
- 进行了基因本体学 (GO) 和KEGG丰富分析.
- 构建蛋白质-蛋白质相互作用 (PPI) 网络和应用机器学习算法.
- 使用接收器操作特征 (ROC) 曲线分析,基因组丰富分析 (GSEA) 和暴露于颗粒物 (PM) 的体外皮肤模型的验证结果.
主要成果:
- 确定了140个常见基因和两个关键诊断基因:FOSL1和TKT.
- ROC分析证实了FOSL1和TKT的诊断值,以及曲线下的最佳面积 (AUC) 值.
- 使用RT-qPCR和Western Blot的实验验证证证了FOSL1和TKT对PM暴露的可靠性,显示皮肤屏障损伤基因的表达增加,具有更高的PM度.
结论:
- 已确定FOSL1和TKT是空气污染引起的皮肤损伤的关键基因和潜在生物标志物.
- 这些发现为了解空气污染对皮肤健康的有害影响提供了分子基础.
- 该研究强调了基于这些已识别的生物标志物进行有针对性的干预的潜力.
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