线粒体和核糖体应激 潜在的前核包膜分解失败:从人类胚胎细胞单细胞转录组学的见解
Chaoying Wang1,2,3, Junnan Fang1,2,3, Guang Yang1,2,3
1Center for Reproductive Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Molecular reproduction and development
|March 6, 2026
概括
在ICSI胚胎中,前核包膜分解 (PNEB) 失败与线粒体功能障碍和核糖体生物发生障碍有关. 这项研究确定了MT-ND1-RPL10A/RPL38轴作为胚胎质量评估的潜在标记.
科学领域:
- 胚胎学 胚胎学
- 分子生物学分子生物学
- 生殖医学 生殖医学
背景情况:
- 核前包膜分解 (PNEB) 失败是细胞内注射精子 (ICSI) 胚胎早期停产的主要原因.
- 导致PNEB失败的分子机制及其对胚胎发育的影响尚未完全理解.
研究的目的:
- 阐明与PNEB失败相关的监管网络.
- 调查PNEB失败对胚胎发育的影响.
- 为了确定ICSI胚胎质量的潜在分子标记物.
主要方法:
- 单细胞测序,以识别PNEB型2PN和3PN胞体之间的差异表达基因 (DEG).
- 重量基因同表达网络分析 (WGCNA) 用于识别与PNEB失败相关的基因模块.
- 最小绝对缩小和选择操作员 (LASSO) 回归用于核心基因查.
- 使用ROS和JC-1光染色对氧化应激和线粒体功能的分析.
主要成果:
- 确定了1294个DEG,包括氧化酸化基因 (MT-ND1,MT-CO3) 的上调和DNA修复基因的下调.
- WGCNA确定了一种与PNEB失败密切相关的浅绿色模块,其中枢纽基因RPL10A和RPL38参与了核糖体生物发生.
- 在PNEB失败组中观察到线粒体代谢功能障碍 (降低JC-1比率) 和升高的ROS水平.
- MT-ND1和RPL10A之间的功能相互作用表明线粒体功能和核糖体组装之间存在联系.
结论:
- 线粒体代谢功能障碍和核糖体组合异常是PNEB失败的关键因素,并随后破坏了核膜稳定性.
- MT-ND1-RPL10A/RPL38轴代表了一种新的潜在分子标志物,用于评估ICSI胚胎质量.
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