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种子中的葡萄杯多样性的演变:专注于Solanaceae中膨胀的果实杯背后的转录基因重塑机制

Wei Gou^1,2,3, Nan Xu^1,2,3, Qiaoru Li^1,2,3

¹State Key Laboratory of Plant Diversity and Specialty Crops/Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.

The Plant journal : for cell and molecular biology

|March 6, 2026

概括

这项研究揭示了恒久性葡萄杯在血管苗中很常见,而生植物的膨胀葡萄杯综合征 (ICS) 代表了一个关键的进化新奇. 分子机制,包括基因表达和沉默,解释了这种独特的水果杯的发展.

科学领域:

植物进化发育生物学植物进化发育生物学
血管精子形态学和基因组学

背景情况:

雄性精种体表现出多样化的形式和功能,但这种多样性的进化路径和分子基础尚未完全理解.
虽然切的玻璃杯是祖先的,但大多数现存的芽种具有持久的玻璃杯,其尺寸有显著的变化.
索拉纳科 (Solanaceae) 家族表现出了显著的的多样性,包括在Physalis中独特的膨胀综合征 (ICS).

研究的目的:

为了研究血管精子葡萄杯的进化史和驱动膨胀葡萄杯综合征 (ICS) 发展的分子机制.
为了确定关键的基因和调节途径,涉及水果果杯的形态和功能进化.

主要方法:

祖先状态的重建,以推断杯的进化模式.
在ICS (Physalis floridana) 和非ICS (Capsicum annuum,Solanum spp.) 之间进行的时间过程转录组比较. 一个物种. 一个物种.
病毒诱导的基因沉默和CRISPR/Cas9将关键基因 (POS4,POS5) 击倒/取消.
对Physalis calyx基因的异种表达分析.

主要成果:

持久性葡萄杯很普遍,具有显著的尺寸变化;Physalis中的ICS是一种独特的进化创新.
转录组分析揭示了异型基因表达和基础ICS的替代拼接的显著变异.
在Physalis中的POS4和POS5基因的淘汰或淘汰显著减少了ICS大小,证实了它们在玻璃杯膨胀中的作用.
在Physalis膨胀杯中的基因被丰富为光合作用和对刺激的反应中的功能,这表明果功能被捕获.

结论:

这项研究阐明了血管精子葡萄杯的进化轨迹,突出了形葡萄杯的祖先性质和持久形式的流行.
转录基因重组,涉及基因表达和拼接变异,驱动了Solanaceae中膨胀的果汁杯的发育和功能演变.
在Physalis中膨胀的果汁杯可能已经选择了最初与果相关的功能,为植物形态演变提供了洞察力.

关键词:

这就是CRISPR/Cas9的作用.这是Solanaceae.卡利克斯 (calyx) 是一个葡萄杯.比较的转录组学进行比较进化演化演化演化演化演化演化演化异质量测量 (heterometry) 是一种测量方法.

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