SARS-COV-2的尖端蛋白增加了CxCR4的表达和乳腺癌细胞在体外的迁移
L Shlapatska1, I Abramenko2, M Zavelevich1
1R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, the National Academy of Sciences of Ukraine, Kyiv, Ukraine.
Experimental oncology
|March 6, 2026
概括
SARS-CoV-2 尖端蛋白 (SP) 可能通过增加细胞迁移和改变关键信号通路来促进乳腺癌 (BC) 转移. 这项研究调查了SPSP.
科学领域:
- 在瘤学瘤学.
- 病毒学 病毒学
- 细胞生物学 细胞生物学
背景情况:
- 建议病毒性呼吸道感染促进乳腺癌 (BC) 转移.
- 导致COVID-19的病毒SARS-CoV-2在触发BC进展及其潜在机制中的作用仍然不清楚.
研究的目的:
- 研究SARS-CoV-2尖端蛋白 (SP) 对BC中关键信号轴组件 (CXCL12/CXCR4) 的表达的影响.
- 评估SP对细胞粘附标记 (CD326,CD54),表皮标记 (cytokeratin-18),β-catenin以及体外迁移活动的影响.
主要方法:
- 乳腺癌细胞系MCF-7和MDA-MB-231与SARS-CoV-2SP进行了48小时的化.
- 标记物表达 (CXCR4,CXCL12,CD326,CD54,细胞素-18,b-catenin) 使用流细胞计量进行量化测量.
- 细胞迁移被评估使用划痕试验.
主要成果:
- 在两种细胞系中,SP显著增加了CXCR4表达.
- SP差异地影响了CXCL12的表达,在MDA-MB-231细胞中增加了它,在MCF-7细胞中减少了它.
- SP治疗改变了粘附分子和细胞素-18的表达,并在两个细胞系中加速迁移,特别是在MDA-MB-231细胞中.
结论:
- 乳腺癌影响与乳腺癌进展相关的免疫类型标记物.
- 观察到的变化表明,SP可能会在MCF-7细胞中诱导上皮层-介质细胞过渡.
- 在研究的两种乳腺癌细胞系中,SP似乎增强了迁移活动.
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