常规和特殊染色技术的比较评估细针吸收淋巴结细胞学中的淋巴结
Ramis Khan1, Areeba Nasar2, Arun Kumar Yadav1
1Pathology, Madhav Prasad Tripathi Medical College, Siddharthnagar, IND.
Cureus
|March 6, 2026
概括
精细针吸入细胞学 (FNAC) 与特殊染料和免疫细胞化学相结合,显著提高了淋巴结感染和癌症的诊断准确性. 奥拉胺-罗达胺染色比齐尔-尼尔森更敏感地检测结核病,有助于在资源有限的环境中早期诊断.
科学领域:
- 病理学 病理学 病理学
- 细胞学 细胞学
- 传染性疾病 传染性疾病
- 在瘤学瘤学.
背景情况:
- 精细针吸入细胞学 (FNAC) 是淋巴腺病的标准诊断工具.
- 恶性瘤和结核病 (TB) 是发展中国家淋巴结扩大的常见原因.
- 常规细胞学染色可能缺乏对某些感染和瘤状况的敏感性.
研究的目的:
- 为了比较常规和特殊染色方法在淋巴结的FNAC中的诊断效果.
- 评估免疫细胞化学与FNAC一起用于淋巴腺病诊断的实用性.
- 为了评估auramine-rhodamine与Ziehl-Neelsen染料在淋巴结吸附体中检测结核病的灵敏度.
主要方法:
- 一项观察性研究涉及104名接受FNAC的淋巴腺病患者.
- 常规 (H&E,Giemsa),特殊 (ZN,AR,PAS) 和免疫细胞化学 (CD15,CD45) 染料的应用.
- 使用千平方测试,科恩卡帕和灵敏度/特异性测量方法进行统计分析.
主要成果:
- 反应性淋巴腺炎是最常见的诊断 (57.7%).
- 在27.8%的可疑结核病例中,Auramine-rhodamine检测到酸性快速细菌,而Ziehl-Neelsen的情况为11.1%.
- 在7.7%的病例中诊断出恶性病变,临床怀疑和细胞学诊断之间有很强的一致性 (科恩的卡帕=0.754).
结论:
- 将FNAC与特殊染料和免疫细胞化学相结合,可以提高感染性和瘤性淋巴腺病的诊断准确度.
- 与Ziehl-Neelsen相比,auramine-rhodamine染色表明结核病检测的灵敏度更高.
- 这种全面的方法对于及时和准确的诊断至关重要,特别是在资源有限的环境中.
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