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During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...

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Updated: Mar 7, 2026

Engineering Antiviral Agents via Surface Plasmon Resonance

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Published on: June 14, 2022

蛋白质链接器联合工程用于针对包裹病毒的宽频抗病毒开发.

Lixia Wei^1,2, Colleen N Loynachan¹, Gregory Mathez³

¹Institute of Materials Science and Engineering, École polytechnique fédérale de Lausanne, Lausanne 1015, Switzerland.

ACS materials letters

|March 6, 2026

概括

科学家们开发了一种新方法,通过修改天然蛋白质,制造出广泛的,无毒的抗病毒药物. 这种多功能平台显示了对当前和未来病毒威胁的快速发展的承诺.

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Use of Viral Entry Assays and Molecular Docking Analysis for the Identification of Antiviral Candidates against Coxsackievirus A16

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Last Updated: Mar 7, 2026

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Published on: June 14, 2022

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科学领域:

生物化学生物化学
病毒学病毒学
药物发现药物发现药物发现

背景情况:

新兴和重新出现的病毒对全球健康构成重大风险.
广泛的抗病毒药物对于疫情准备至关重要.
现有的抗病毒策略面临着耐药性和特异性的挑战.

研究的目的:

开发一种通用和简单的战略,用于制造广泛的,无毒的抗病毒药物.
将天然蛋白质功能化为有效的抗病毒化合物.
评估这些修饰蛋白对包裹病毒的疗效和机制.

主要方法:

自然蛋白质的化学功能化通过通过二次胺终结的基连接物的一步结合.
在体外测试功能化蛋白质对疹简单病毒2 (HSV-2),流感A H1N1和SARS-CoV-2的测试.
评估抗病毒活性,连接体密度影响,基链长度影响和血清环境中的性能.

主要成果:

功能化的蛋白质显示出对HSV-2,流感A H1N1和SARS-CoV-2的强有力的抑制 (在纳米到微分子范围内EC50).
抗病毒疗效与配体密度和基链长度正相关.
较长的基链将活性从可逆性 (病毒静态) 转移到不可逆性 (病毒杀伤性).
这些抗病毒化合物在复杂的血清环境中保持了强大的性能,并且在预防方面最有效.

结论:

一个多功能和简单的蛋白质功能化策略产生了广泛的,无毒的抗病毒药物.
这个平台提供了一种有前途的方法,用于快速开发抗病毒,以对抗各种病毒威胁.
修改后的蛋白质提供了对新兴和重新出现的病毒的可行的第一道防线.