人类乳头瘤病毒阳性疾病的循环瘤DNA:准备好了吗?
Émilie C M de Groot1,2, C René Leemans1,2, Ruud H Brakenhoff1,2
1Amsterdam UMC location Vrije Universiteit Amsterdam, Department of Otolaryngology/Head and Neck Surgery.
Current opinion in oncology
|March 6, 2026
概括
液体活检显示出对监测人类乳头瘤病毒阳性口腔癌的前景. 需要进一步的研究来规范ctHPV-DNA检测方法用于早期复发诊断.
科学领域:
- 在瘤学瘤学.
- 分子诊断学 分子诊断
- 病毒学 病毒学
背景情况:
- 与人类乳头瘤病毒 (HPV) 相关的口腔状细胞癌 (OPSCC) 正在增加.
- 目前的监测依赖于治疗后3-4个月的成像 (MRI/PET-CT),其中10-15%的患者经历了残留疾病或晚期复发.
- 这些局限性需要先进的方法来及时检测和管理.
研究的目的:
- 审查HPV阳性OPSCC中液体活检的应用,用于早期检测和疾病监测.
- 探索在血中检测循环瘤HPV DNA (ctHPV-DNA) 的潜力,以早期诊断复发.
- 评估分子诊断方法在临床实践中的现状.
主要方法:
- 对使用滴滴数字PCR (ddPCR) 和DNA测序用于ctHPV-DNA检测的已发表研究的综述.
- 对目前HPV阳性OPSCC监测方法及其局限性的分析.
- 关于在血中利用ctHPV-DNA用于复发的早期分子诊断的建议.
主要成果:
- 各种研究已经探索了对HPV阳性OPSCC应用的ddPCR和DNA测序.
- ddPCR和DNA测序都有优点和局限性,其适用性取决于具体的用例.
- 目前的试验缺乏标准化,发表的研究往往很小.
结论:
- 使用HPV阳性OPSCC的液体活检进行疾病监测具有显著的前景.
- 测试的标准化和大规模的临床研究比较ddPCR和DNA测序至关重要.
- 在广泛采用之前,需要开发一种标准化,临床适用的方法.
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