综合的多omics描述剖析在喉癌的第二个原发性肺癌的发展:一个观察性研究
Fengfeng Xu1, Tengfei Huang1, Qianhui Xu1
1Department of Cardiothoracic Surgery, 900 Hospital of the Joint Logistic Team, Fuzhou, People's Republic of China.
喉癌患者的第二次原发性肺癌 (SPLC) 涉及共享的基因和改变的免疫细胞. 单细胞中CXCL12介导的新陈代谢可能推动SPLC的发展,提供新的治疗点.
科学领域:
- 在瘤学瘤学.
- 基因组学就是基因组学.
- 免疫学 免疫学 免疫学
背景情况:
- 喉癌具有显著的死亡风险,通常是由于第二次原发性肺癌 (SPLC) 的发展.
- 了解驱动喉癌患者SPLC的分子机制对于改善患者的治疗结果至关重要.
研究的目的:
- 调查喉癌患者中SPLC发展的潜在机制.
- 为了确定与SPLC相关的共享遗传变化,调节网络和代谢途径.
主要方法:
- 喉癌和肺癌数据集的综合多奥米克分析 (GSE51985,GSE102287).
- 使用"limma"包的差异基因表达分析.
- 转录因子 (TF) -miRNA-基因相互作用网络的构建.
- 代谢途径分析和单细胞RNA测序 (GSE150321,GSE127471) 的研究.
- 使用CIBERSORT进行免疫细胞透分析.
主要成果:
- 在喉癌和肺癌之间确定了9个共享的差异表达基因,包括上调的UBE2C,POLQ,RAD51,HOXB7和下调的EDNRB,GPD1L,F10,SORBS2,CXCL12.
- 功能性丰富性分析强调了参与癌症途径和细胞循环的作用.
- 构建了TF-miRNA基因网络,涉及120个TF和246个miRNA.
- CXCL12与伊诺西酸代谢有关,在肺癌中介性单细胞中观察到高活性.
- 与非瘤组织相比,在两种癌症类型中都发现了巨细胞透的增加.
结论:
- 由特定的TFs和miRNAs协调的共享遗传改变,有助于SPLC的发展.
- 改变的免疫微环境,特别是巨细胞的增加,起到了作用.
- 通过CXCL12介导的内醇酸盐代谢,可能由中间单细胞驱动,与SPLC的发病有关.
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