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Updated: Mar 8, 2026

Competitive Genomic Screens of Barcoded Yeast Libraries
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Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

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康尼斯 (ConNIS) 和标签不稳定性:新的统计方法可以提高TraDIS库中基本基因的检测

Moritz Hanke1, Theresa Harten2, Ronja Foraita1

  • 1Department Statistical Methods in Epidemiology, Leibniz Institute for Prevention Research and Epidemiology - BIPS, Bremen, Bremen, Germany.

PLoS computational biology
|March 6, 2026
PubMed
概括

一种名为连续非插入位点 (ConNIS) 的新方法可以从转子子定向插入位点测序 (TraDIS) 数据中准确识别基本基因. 康尼斯为基因基本性确定提供了统计基础,改进了现有的方法,特别是低插入密度的方法.

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Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity

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Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine10:40

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

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Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
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Significance Testing: Overview

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Quantifying and Rejecting Outliers: The Grubbs Test01:02

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Sometimes, a data set can have a recorded numerical observation that greatly  deviates from the rest of the data. Assuming that the data is normally distributed, a statistical method called the Grubbs test can be used to determine whether the observation is truly an outlier.  To perform a two-tailed Grubbs test, first, calculate the absolute difference between the outlier and the mean. Then, calculate the ratio between this difference and the standard deviation of the sample. This...
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