Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

The average temperature of Earth is the subject of much current discussion. Earth is in radiative contact with both the Sun and dark space; it receives almost all its energy from the radiation of the Sun and reflects some of it into outer space. Dark space is very cold, about 3 K, so Earth radiates energy into it. For instance, heat transfer occurs from soil and grasses, the rate of which can be so rapid that frost can occur on clear summer evenings, even in warm latitudes.
The average...

There are three methods by which heat transfer can take place: conduction, convection, and radiation. Each method has unique and interesting characteristics, but all three have two things in common: they transfer heat solely because of a temperature difference; and the greater the temperature difference, the faster the heat transfer.
In order to solve a problem related to heat transfer, first of all, the situation needs to be examined to determine the type of heat transfer involved. This could...

The rate of heat transfer by emitted radiation is described by the Stefan-Boltzmann law of radiation: