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A Multi-detection Assay for Malaria Transmitting Mosquitoes
Published on: February 28, 2015
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两种不同的VAR2CSA血统,具有不同的进化特征,在泰国的plasmodium falciparum中循环
Sethawud Chaikitgosiyakul1, Napaporn Kuamsab2, Taweesak Tia3
1Molecular Biology of Malaria and Opportunistic Parasites Research Unit, Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; Inter-Department Program of Biomedical Sciences, Faculty of Graduate School, Chulalongkorn University, Bangkok, Thailand.
概括
在泰国流传着两种不同的Plasmodium falciparum VAR2CSA血统,阻碍了疫苗的开发. 了解这种遗传多样性对于制造有效的胎盘疟疾疫苗至关重要.
科学领域:
- 遗传学 是一个遗传学.
- 免疫学 免疫学 免疫学
- 寄生虫学的寄生虫学
背景情况:
- 由Plasmodium falciparum引起的胎盘疟疾对孕妇和胎儿构成重大威胁.
- VAR2CSA是胎盘疟疾疫苗的关键目标,但其遗传多样性使疫苗设计复杂化.
- 从临床分离物中获得的有限的全长VAR2CSA序列数据限制了我们对其多态性的理解.
研究的目的:
- 鉴定泰国的症状性Plasmodium falciparum分离物中VAR2CSA全长基因序列的特征.
- 调查该地区VAR2CSA的遗传多样性和进化模式.
- 为改善疫苗开发提供关于VAR2CSA双部分变异的见解.
主要方法:
- 从泰国五个省份收集了31个有症状的Plasmodium falciparum分离物.
- 使用远程PCR和桑格测序确定了完整的var2csa编码序列 (9.1-9.9 kb).
- 分析了在基因区域中对哈普洛型多样性,遗传学关系和核酸多样性的序列数据.
主要成果:
- 确定了24个不同的VAR2CSA单体类型,具有高度多样性 (h ± S.D. = 0.97 ± 0.02) 的情况.
- 揭示了两个主要的循环血统:一个主导的3D7类型和一个明显的DD2类型.
- 与5'区域相比,在3'区域 (DBL6ε到ATS) 中观察到显著更高的核酸多样性,这是由于两种谱系之间的深度分歧所驱动的.
结论:
- 在泰国,两种具有明显进化特征的两种截然不同的VAR2CSA血统共同流传.
- Dd2型血统表现出独特的特征,包括扩展的ID4区域和极端的3'端分歧.
- 了解这种双重遗传变异对于开发针对胎盘疟疾的基于VAR2CSA的广泛保护性疫苗至关重要.
关键词:
不同的血统不同.全长度的 var2csa 序列.疟疾:疟疾是一种疾病.胎盘疟疾是胎盘疟疾的一种疾病.原菌 (Plasmodium falciparum) 是一种有毒的病毒.再组合的重组方式这就是VAR2CSA.更多相关视频
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