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神经介质用于单细胞蛋白质生产:通过介质优化和大气和室温等离子体突变发生的增强

Haifeng Zhao¹, Shichuang Sun¹, Mengru Jin¹

¹College of Light Industry, Liaoning University, Shenyang 110036, China.

Bioresource technology

|March 6, 2026

概括

中间神经被改造成一种高蛋白菌株,T154,使用大气和室温等离子体 (ARTP) 突变发生. 这种优化的菌株有效地生产可持续的单细胞蛋白质,用于肉类替代品的潜在用途.

科学领域:

生物技术是生物技术.
食品科学食品科学食品科学
微生物工程微生物工程

背景情况:

神经介质有效地将低成本基质转化为单细胞蛋白质.
对Neurospora intermedia的高密度培养策略尚不清楚,这限制了它的潜力.

研究的目的:

为了开发一种高蛋白产生的Neurospora中间突变体.
为高密度生长优化种植条件.
评估工程菌株对可持续蛋白质生产的潜力.

主要方法:

大气和室温等离子体 (ARTP) 突变发生被用来从神经介质FF171.1.中提取T154突变体.
使用糖蜜和Box-Behnken设计进行了中等优化.
分析了生物质产量,蛋白质含量和肉类类型的特性.

主要成果:

突变T154表现出稳定的遗传特征,并达到大约60g/L的干细胞重量.
中等优化增加了蛋白质产量,从18.0g/L增加到28.9g/L.
T154菌株的最终蛋白质产量达到了35.1g/L,比单纯的中等优化提高了22%.
来自T154生物质的肉类类型显示了较低的水损失 (35%) 和改善的纹理.

结论:

成功建立了Neurospora intermedia的高密度培养策略.
改造的T154菌株显示出作为可持续的替代蛋白质来源的巨大潜力.
这项研究为在工业规模的蛋白质生产中利用Neurospora intermedia提供了基础.

关键词:

替代蛋白质是一种替代蛋白质.状真菌是一种状真菌.食品级的基蛋白是食品级的基蛋白.低成本的基板是低成本的肉类模拟器肉类模拟器血诱导的突变发生.

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