相关实验视频

Updated: Mar 8, 2026

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Published on: September 9, 2012

在凝血中提前精确诊断:引入和分析验证基于多重质谱的测试,用于五个凝血因子

Noa A Linthorst¹, Richard J Dirven¹, Fred P H T M Romijn²

¹Department of Internal Medicine, Division of Thrombosis and Hemostasis, Einthoven Laboratory for Vascular and Regenerative Medicine, Leiden University Medical Center, Leiden, The Netherlands.

Journal of thrombosis and haemostasis : JTH

|March 6, 2026

概括

定量蛋白质质谱法 (QPMS) 为测量纤维素素 (FBG) 和·威尔布兰德因子 (VWF) 等关键凝血蛋白提供了一种多重方法. 这种经过验证的QPMS测试对临床诊断有希望,尽管因子VIII (FVIII) 需要丰富.

科学领域:

临床化学临床化学
蛋白质组学是指蛋白质组学.
生物技术是生物技术.

背景情况:

凝血和纤维解蛋白对静血至关重要,但传统的测定方法有限.
现有的方法缺乏多重复合,标准化,并且需要大量的等离子体.
定量蛋白质质谱法 (QPMS) 提供了多重蛋白质量测量的解决方案.

研究的目的:

开发和验证QPMS试验,用于同时测量五种关键的凝血蛋白.
解决传统试验在吞吐量和样本量方面的局限性.

主要方法:

一个QPMS测试旨在量化血纤维素素 (FBG),·威尔布兰德因子 (VWF),因子VIII (FVIII),因子IX (FIX) 和因子XI (FXI).
用于精确量化,使用了代表性的三性和稳定同位素标记的内部标准.
分析验证包括对精度,线性,特异性,稳定性,转移,参考间隔,方法比较和矩阵效应的评估.

主要成果:

在和EDTA血中,QPMS成功量化了FBG,VWF,FIX和FXI.
该试验显示出高精度 (3.4-6.7%变化) 和线性 (r=0.990-0.998),与传统方法非常一致.
在多次冷解周期后,蛋白质信号保持稳定,这表明了测试的稳定性.

结论:

一个经过验证的QPMS试验可以实现FBG,VWF,FIX和FXI的多重定量.
富化策略是必要的,以准确的FVIII测量,因为它的低丰度.
这些发现支持QPMS用于凝血诊断的分析可行性,以及未来开发全面的血液静止面板.

关键词:

血液凝固因子血液凝固因子第九个因子是因子IX.第八个因子十一号因素十一号因素纤维素原蛋白是一种纤维素.量化质谱法量化质谱法量化质谱法 ·维勒布兰德因素是什么

更多相关视频

Author Spotlight: Deciphering Coagulation Disorders in Traumatic Brain Injury Patients

Author Spotlight: Deciphering Coagulation Disorders in Traumatic Brain Injury Patients

Published on: August 4, 2023

Leveraging Turbidity and Thromboelastography for Complementary Clot Characterization

Leveraging Turbidity and Thromboelastography for Complementary Clot Characterization

Published on: June 4, 2020

相关概念视频

Coagulation

Coagulation

Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...

Coagulation

Coagulation

The coagulation phase is a critical part of the body's process to prevent blood loss following injury to blood vessels. It involves chemical reactions that form a clot to seal the injured area. The clotting process begins shortly after injury, within 15-20 seconds for severe damage and 1-2 minutes for minor injuries.
During the coagulation phase, clotting factors, or procoagulants, play a vital role in initiating and progressing the coagulation cascade. This cascade is a series of reactions...