微生物诱导的酸盐沉由本土的性细菌:一种双酶策略,用于在水泥材料中的裂纹愈合
Mohammad Shiri1, Abbas Bahari2, Mohammad Mahdi Khani Sarbangholi1
1Department of Civil Engineering, University of Zanjan, Zanjan, Iran.
Applied microbiology and biotechnology
|March 6, 2026
概括
研究人员对来自极端环境的200多种细菌进行了选,以确定它们产生酸盐的能力. 通过微生物诱导的石沉,确定了四种强壮的菌株,可以通过微生物诱导的石沉来开发耐用,自我愈合的混凝土.
科学领域:
- 土木工程 土木工程是指土木工程.
- 微生物学 微生物学
- 材料科学 材料科学 材料科学
背景情况:
- 微生物诱导的石沉 (MICP) 是一种可持续的混凝土裂修复方法.
- 水泥中的高性限制了微生物对自愈混凝土的有效性.
研究的目的:
- 选和选择具有双尿素酶和碳酸酶 (CA) 活性的性细菌,用于自愈混凝土.
- 开发一个定量框架来评估细菌化潜力.
主要方法:
- 对来自极端环境的200多种本土细菌分离物的选.
- 评估尿酶和碳酸酶 (CA) 的活性,并制定酶活性指数 (EAI).
- 在性条件下 (pH 13.5) 评估细菌存活率,化和CaCO3沉.
主要成果:
- 四种形成子的菌株 (Bacillus subtilis,Sporosarcina pasteurii,Bacillus sphaericus,E10.2) 显示出高的EAI和性耐受性.
- 选择的细菌保留了>70%的酶活性,并沉了高达89%的CaCO3.
- 生物处理的砂表现出增强的强度 (46.8 MPa) 和裂愈合,由UPV,SEM和XRD证实.
结论:
- 建立了一个基于绩效的框架,用于为自愈混凝土选择性细菌.
- 产生双酶的细菌显著提高了混凝土的耐用性和自主裂修复能力.
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