CPK8-AHA1酸化模块抑制了血膜的H+-ATPase活性,从而赋予干旱耐受性
Xiao Liu1, Dongmin Zhang1, Yongqing Yang1
1State Key Laboratory of Plant Environmental Resilience (SKLPER), College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Plant physiology
|March 7, 2026
概括
植物对干旱的耐受性通过依赖性酶8 (CPK8) 酸化血膜质子 (H+) -ATPase而增强. 这种机制微调口腔关闭,减少干旱压力期间的水损失.
科学领域:
- 植物生理学 植物生理学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 血膜 (PM) 质子 (H+) - ATPase 调节植物生长和应激反应.
- 抑制PM H+-ATPase促进口腔关闭,减少干旱条件下的水损失.
- (Ca2+) 信号调节PM H+-ATPase活性的机制尚未完全理解.
研究的目的:
- 阐明Ca2+信号调节PM H+-ATPase活性的机制.
- 为了确定参与调节PM H+-ATPase活性的特定激酶.
- 研究这种调节途径在植物干旱耐受性中的作用.
主要方法:
- 同免疫沉试验证实了CPK8和AHA1.1之间的物理相互作用.
- 在体外激酶测试以确定酸化部位和对AHA1活性的影响.
- 在干旱压力下的CPK8过度表达和ost2-2D突变系中分析植物表型 (透气流失水分,叶子温度).
- 在干旱压力期间监测Ca2+信号和CPK8激活.
主要成果:
- 阿拉比多普西斯的依基因酶8 (CPK8) 与血膜质子酶1 (AHA1) 在Ser-899.9上物理相互作用并酸化.
- 通过CPK8介导的酸化显著抑制了AHA1 H+-ATPase的活性.
- 过度表达CPK8改善了ost2-2D突变体的过度水损失和叶子温度的增加.
- 干旱引起的Ca2+信号激活CPK8,调节PM H+-ATPase活动.
结论:
- 通过酸化,CPK8起到PM H+-ATPase活动的关键调节者的作用.
- 这种CPK8-AHA1通路对于微调口腔关闭至关重要,以应对干旱压力.
- 这些发现揭示了优化植物耐旱能力的新机制.
相关概念视频
01:57Adaptations that Reduce Water Loss
28.4K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
28.4K
02:04Regulation of Transpiration by Stomata
31.8K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
31.8K
01:25Cell Signaling in Plants
6.9K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
6.9K
01:19Allosteric Proteins-ATCase
6.8K
Binding sites linkages can regulate a protein's function. For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
6.8K
01:27ATP Driven Pumps I: An Overview
10.1K
ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
10.1K
02:41Responses to Drought and Flooding
12.2K
Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.
12.2K