相关实验视频

Updated: Mar 9, 2026

Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment

Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment

Published on: November 23, 2017

造血性PI3Kγ通过调节巨细胞贩运来促进腹腔大动脉动脉瘤

Shuai Liu¹, Yu Liu², Yacheng Xiong²

¹Department of General and Vascular Surgery, Xiangya Hospital, Central South University, Changsha 410008, China; Xiamen Cardiovascular Hospital, Xiamen University, Xiamen City, Fujian Province, China.

Journal of molecular and cellular cardiology

|March 7, 2026

概括

在骨髓细胞中准PI3Kγ可以通过控制巨细胞迁移来治疗腹腔大动脉动脉瘤 (AAA). 这条通路调节炎症和大动脉扩张,为AAA疾病提供了潜在的治疗策略.

科学领域:

心血管生物学心血管生物学
免疫学免疫学免疫学
疾病的分子机制.

背景情况:

腹腔大动脉瘤 (AAA) 涉及由炎症驱动的渐进性大动脉扩张.
酸酸3-激酶 (PI3Kγ) 对于心血管疾病中髓状细胞炎症反应至关重要.

研究的目的:

研究PI3Kγ在促进AAA进展中的作用和机制.
确定PI3Kγ抑制是否可以成为AAA的治疗策略.

主要方法:

在弹性酶或血管新生素II诱导的AAA模型中使用了野生类型,PI3Kγ-knockout和骨髓转移的小鼠.
评估巨细胞迁移和招募使用体内,腹膜和透孔测试.
在骨髓衍生的巨细胞 (BMDMs) 上进行了体外研究,以分析PI3Kγ对自流和迁移的影响,采用免疫沉和无处不在的免疫破坏.

主要成果:

通过减弱单细胞迁移,PI3Kγ缺乏抑制了实验AAA,减少了弹性质的破坏和造血细胞透.
血液生成PI3Kγ被证实是通过骨髓移植对AAA形成和进展至关重要的.
抑制PI3Kγ通过影响MLCK泛化和MLC酸化,损害了巨细胞迁移,并促进了MLC的自降解,这对于调节巨细胞迁移持久性至关重要.

结论:

造血性PI3Kγ通过调节巨细胞迁移,通过受乌比基调节的MLCK周转和自性MLC降解来驱动AAA病变.
药理上抑制PI3Kγ为临床AAA治疗提供了一个有前途的治疗途径.

关键词:

腹腔大动脉动脉瘤腹腔大动脉动脉瘤炎症炎症是一种炎症.巨细胞迁移的情况肌酸氨酸轻链肌酸氨酸轻链 PI3Kγγ 的意思是

更多相关视频

A Calcium Phosphate-Induced Mouse Abdominal Aortic Aneurysm Model

A Calcium Phosphate-Induced Mouse Abdominal Aortic Aneurysm Model

Published on: November 18, 2022

Mouse Model of Alloimmune-induced Vascular Rejection and Transplant Arteriosclerosis

Mouse Model of Alloimmune-induced Vascular Rejection and Transplant Arteriosclerosis

Published on: May 17, 2015

相关概念视频

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...