相关实验视频

Updated: Mar 9, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

染色体尺度的基因组组合和注释Rosa bracteata (马卡特尼) 的基因组

Runhui Li^1,2, Yanhong He^1,2, Fayun Xiang³

¹National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan, 430070, China.

Scientific data

|March 7, 2026

概括

研究人员介绍了野生物种Rosa bracteata的第一个高质量基因组组. 这种有价值的资源有助于了解的遗传学,耐热性,并推进育种计划.

科学领域:

基因组学就是基因组学.
植物科学植物科学
进化生物学进化生物学

背景情况:

罗莎·布拉克泰塔 (Rosa bracteata) 是罗莎科布拉克泰塔 (Rosa section Bracteatae) 中唯一的野生物种.
目前还没有对这一部分的基因组组合,这限制了研究.
在繁殖中,R. bracteata因其耐热性而至关重要.

研究的目的:

为了生成高质量的,染色体尺度的基因组组合罗莎骨干.
为R. bracteata研究提供一个基础资源.
促进对的遗传进化和适应性特征的研究.

主要方法:

使用了高通量测序技术.
生物信息工具被用于组装和基因预测.
基因组组装被固定在七个伪染色体中.

主要成果:

实现了540.63 Mb的染色体规模基因组组装.
连接N50达到35.97Mb,表明连接性很高.
预测了42,789个编码蛋白质的基因,其中38,394个功能性注释.

结论:

R. bracteata 的基因组组合是基因组学的一个重大进步.
这种资源将使热耐受性和遗传多样性的研究成为可能.
它将加速分子育种的努力,以改善品种.

更多相关视频

A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants

A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants

Published on: December 16, 2015

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

Published on: June 28, 2012

相关概念视频

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

Lampbrush Chromosomes

Lampbrush Chromosomes

In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...