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Updated: Mar 9, 2026

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy
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在单链和双链RNA内部,光尿素 (qU) 的亮度增加

Alma F E Karlsson1, Pauline Pfeiffer1, Hoang-Ngoan Le1,2

  • 1Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Kemivägen 10, Gothenburg, SE-41296, Sweden.

Scientific reports
|March 7, 2026
PubMed
概括

纳入RNA的四环尿素 (qU) 显示了增强的光量子产量和更长的寿命. 这种明亮的光基类比对光显微镜等应用非常有价值.

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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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