Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A catalyst and base-free electrochemical <i>ortho</i>-amination of phenols.

Chemical communications (Cambridge, England)·2026
Same author

Eutectogel-Based Antifouling Interface: Addressing Sensitivity and Accuracy Challenges in Complex Serum.

Analytical chemistry·2026
Same author

Fragment-Imprinted Synergistic Copper Ion-Bridged Silver Nanocomposites Unlock Signal Activation for Picomolar-Level Sensing of l-Histidine.

ACS applied materials & interfaces·2026
Same author

"Near-zero background signal" sensing platform for tumor marker based on catalytic amplification strategy in chronocoulometry.

Talanta·2026
Same author

Highly water-retaining conductive hydrogels based on multi-crosslinked networks for ultrasensitive sensing platform.

Bioelectrochemistry (Amsterdam, Netherlands)·2025
Same author

"AND" logic gate recognition interface for phospholipids based on fragment imprinting.

Journal of colloid and interface science·2025

相关实验视频

Updated: May 11, 2026

Polymer Microarrays for High Throughput Discovery of Biomaterials
13:37

Polymer Microarrays for High Throughput Discovery of Biomaterials

Published on: January 25, 2012

15.1K

基于分子打印标签的小分子识别.

Zhaoxuanxuan Chen1, Ziwei Wang1, Hongliang Han1

  • 1Department of Chemistry, Capital Normal University, Beijing 100048, China.

Journal of colloid and interface science
|March 8, 2026
PubMed
概括

这项研究引入了一种用于特定L-氨酸标签的新型分子印记方法,克服了小分子识别方面的挑战. 开发的纳米球探测器为生物医学应用提供了灵敏有效的解决方案.

科学领域:

  • 生物医学工程 生物医学工程
  • 分析化学 分析化学
  • 分子生物学分子生物学

背景情况:

  • 在生物医学研究中,对小分子的特定识别和标记至关重要.
  • 现有使用aptamer的方法在获取识别单元方面面临挑战.
  • 需要多功能和高效的小分子标签策略.

研究的目的:

  • 开发一种创新的分子印记识别策略,用于小分子的特定标签.
  • 创建一种简单而通用的方法来合成纳米圈探针用于小分子识别.
  • 为了证明这种方法的可行性,使用L-氨酸作为模型分析.

主要方法:

  • 利用分子印记技术 (MIT) 构建基于部分分子识别的分子印记标签 (MIL).
  • 合成的纳米球探 incorporating MIL用于选择性结合.
  • 通过将L-氨酸的一端固定在电极基板上以检测另一端,开发了一种电化学传感器.

主要成果:

  • 成功准备了MIL探针,具有针对L-氨酸的特定识别能力.
  • 达到了12.02 pM的L-氨酸的检测极限 (LOD).
  • 为电化学传感器建立了从1nM到1mM的线性范围.
关键词:
电化学传感接口接口 电化学传感接口分子印记标签的标签.小分子识别小分子识别

更多相关视频

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes
09:28

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

Published on: January 10, 2017

8.6K
Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

12.7K

相关实验视频

Last Updated: May 11, 2026

Polymer Microarrays for High Throughput Discovery of Biomaterials
13:37

Polymer Microarrays for High Throughput Discovery of Biomaterials

Published on: January 25, 2012

15.1K
Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes
09:28

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

Published on: January 10, 2017

8.6K
Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

12.7K

结论:

  • 拟议的分子印记策略为小分子识别和标记提供了可行和有效的解决方案.
  • 这种方法为物质标签和相关领域的分子研究提供了一个一般概念.
  • 开发的纳米球探测器对于检测L-氨酸具有很高的灵敏度和特异性.