相关实验视频

Updated: Sep 11, 2026

Bottom-up and Shotgun Proteomics to Identify a Comprehensive Cochlear Proteome

Bottom-up and Shotgun Proteomics to Identify a Comprehensive Cochlear Proteome

Published on: March 7, 2014

使用iTRAQ和PRM方法进行状血管损伤和PVM/Ms相关修复的蛋白质学表征

Qin Wang¹, Tong Zhang¹, Chaoying Tang²

¹Senior Department of Otolaryngology Head and Neck Surgery, The 6th Medical Center of Chinese PLA General Hospital, State Key Laboratory of Hearing and Balance Science, Key Laboratory of Hearing Science, Ministry of Education, Beijing Key Laboratory of Hearing Impairment Prevention and Treatment, Beijing, China.

Acta oto-laryngologica

|March 8, 2026

概括

噪音暴露会破坏血液迷宫屏障,破坏细胞结合点和周围血管巨细胞类黑色素细胞. 这项研究揭示了一个协调的分子轴驱动屏障破坏和修复.

科学领域:

耳鼻喉科耳鼻喉科耳鼻喉科
神经科学是一个神经科学.
细胞生物学细胞生物学

背景情况:

暴露于噪音 (NE) 破坏了血液迷宫屏障 (BLB),导致毛细胞脱落.
这种干扰涉及到细胞结节和周血管巨细胞类黑色素细胞 (PVM/Ms) 的损伤.

研究的目的:

调查血管内皮生长因子 (VEGF) 和色素表皮衍生因子 (PEDF) 信号在NE后BLB分解和修复中的作用.
了解PVM/Ms功能障碍对BLB完整性在NE之后的贡献.

主要方法:

使用 iTRAQ 和 PRM 在对照小鼠和 NE 小鼠中对血管 (SV) 的蛋白质基因分析.
免疫光,qPCR和其他技术评估了PVM/Ms分布,VEGF/PEDF信号传递和结点蛋白.

主要成果:

确定了9种改变的蛋白质,映射到结节稳定性,代谢调节,离子稳态和免疫调节.
NE导致了急性PVM/Ms减少和VEGF,PEDF和结合蛋白的早期下调.
VEGF/VEGFR2和PEDF/VEGFR1的信号动态与VE-Cadherin,ZO-1和Occludin等关键结点蛋白质的恢复并行.

结论:

暴露于噪音会触发PVM/Ms-VEGF/PEDF-连接蛋白轴的时间依赖性干扰.
这个轴对于血液迷宫屏障的不稳定性和随后的修复至关重要.

关键词:

没有,没有,没有,没有.血管 (Stria vascularis) 是一个血管.对于相对和绝对量化的等离子标签.平行反应监测并行反应监测.周周血管巨细胞类黑色素细胞.

更多相关视频

Author Spotlight: Development of a Laser-Induced Shock Wave Animal Model Without Tympanic Membrane Perforation

Author Spotlight: Development of a Laser-Induced Shock Wave Animal Model Without Tympanic Membrane Perforation

Published on: March 1, 2024

Microfluidic Chip for Axonal Injury Models Construction and Enabling Multi-Omics Analysis

Microfluidic Chip for Axonal Injury Models Construction and Enabling Multi-Omics Analysis

Published on: October 14, 2025

相关概念视频

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...