相关实验视频

Updated: Mar 10, 2026

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

Published on: February 17, 2023

RNA结合蛋白TDP-43和FUS促进R环分辨率并调节转录终止

Dorothy Yanling Zhao¹, Syed Nabeel-Shah¹, Zuyao Ni²

¹The Donnelly Centre, University of Toronto, Toronto, ON, M5S 3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, M5G 1M1, Canada.

The Journal of biological chemistry

|March 9, 2026

概括

TDP-43和FUS蛋白质有助于通过R1810me2s-SMN通路在转录终止期间解决R循环. 它们的功能障碍会影响终止,导致DNA损伤,并可能导致神经退行性疾病,如ALS和FTD.

科学领域:

分子生物学分子生物学
神经科学是一个神经科学.
遗传学是一个遗传学.

背景情况:

TDP-43和FUS是与神经退行性疾病相关的RNA结合蛋白.
功能障碍的RNA处理与ALS和FTD等疾病有关.
R循环与基因组不稳定性和疾病有关.

研究的目的:

研究TDP-43和FUS在转录终结中的作用.
阐明R1810me2s-SMN通路在TDP-43和FUS功能中的参与.
建立TDP-43/FUS,R循环分辨率和神经退行症之间的机制联系.

主要方法:

染色体招募试验用于研究TDP-43和FUS.
RNAPII终止测试用于评估终止效率.
全转录组分析以确定TDP-43结合点和R循环形成.
测试以评估RNA结合活性对TDP-43功能的影响.

主要成果:

通过R1810me2s-SMN通路,TDP-43和FUS被招募到染色质中,促进转录终止.
这种途径的破坏会导致缺陷的RNAPII终止,R循环积累和DNA损伤.
由于其在R循环解析和转录终止中的作用,TDP-43RNA结合至关重要.
TDP-43的结合点与R环形成区域相关.

结论:

在通过R1810me2s-SMN通路的转录终结中,TDP-43和FUS起着至关重要的作用.
由TDP-43/FUS损害的R环分辨率有助于基因组的不稳定性.
这些发现为ALS和FTD的病原体提供了见解.

关键词:

这就是ALS.这就是FUS FUS.在R-Loop中使用.这是一个SMNSMN.在TDP-43中使用.神经退行性疾病的神经退行性疾病转录终止转录终止

更多相关视频

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

相关概念视频

Transcriptional Regulation: Riboswitches

Transcriptional Regulation: Riboswitches

Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...

Transcription Elongation Factors

Transcription Elongation Factors

Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...

Transcription Attenuation in Prokaryotes

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...

Eukaryotic RNA Polymerases

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...

RNA Structure

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...

RNA Structure

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...