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青少年暴露于环境银纳米颗粒诱导精子生成障碍和塞尔托利细胞功能障碍:整合性单细胞转录组学,代谢组学和机械剖析
Jiaochen Luan1, Tong Chen1, Chao Yang2
1Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, PR China; Department of Urology, The Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, Nanjing, PR China.
Ecotoxicology and environmental safety
|March 9, 2026
概括
银纳米粒子 (AgNPs) 通过破坏丸细胞和破坏关键代谢通路来损害男性生育能力. 这项研究揭示了AgNP穿过血屏障,导致细胞特异性毒性,并通过线粒体功能障碍和线粒体衰变影响精子生成.
科学领域:
- 生殖毒理学 生殖毒理学
- 纳米医学是一种纳米医学.
- 细胞生物学 细胞生物学
背景情况:
- 银纳米粒子 (AgNPs) 的广泛使用引发了人们对男性生殖健康的担忧.
- 对丸中AgNP毒性机制缺乏详细的单细胞理解.
研究的目的:
- 阐明AgNP引起的男性生殖毒性背后的细胞和分子机制.
- 为了确定特定的丸细胞类型易受AgNPs.
- 探索潜在的治疗策略,对抗AgNP诱导的不孕症.
主要方法:
- 在青春期老鼠口服暴露AgNPs35天.
- 单细胞转录组学,代谢组学和功能分析.
- 在体外研究中使用丸细胞系和人类初级塞尔托利细胞.
主要成果:
- AgNP 穿越了血液-丸屏障,并在丸中积累,影响生育能力.
- 精子细胞和塞尔托利细胞表现出高敏感性; 不分化的精子细胞干细胞减少.
- AgNPs诱导了细胞特异性毒性,包括抑制增殖,增加亡,氧化应激,并破坏了塞尔托利细胞功能.
- 机制涉及线粒体功能障碍,谷氨代谢中断,以及PINK1/帕金斯介导的线粒细胞衰变.
结论:
- 通过多方面的细胞损伤和代谢干扰,AgNP诱导男性生殖毒性.
- 塞尔托利细胞平衡受到干扰,危及丸支功能.
- 结果为开发对抗纳米粒子诱导的男性不孕症的对策提供了洞察力.
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