五天内冷藏尿样中的Bense Jones蛋白的稳定性:一个分析前评估
Maria Rita Cozzi1, Fabio Del Ben1, Martina Amato1
1Department of Translational Researsuch and Advanced Tumor Diagnostics, Immunopathology and Cancer Biomarkers, CRO Aviano, National Cancer Institute, IRCCS, Aviano, Italy.
Diagnosis (Berlin, Germany)
|March 9, 2026
概括
冷藏尿中的Bense Jones蛋白 (BJP) 在5天内保持稳定,其度变化最小. 这种稳定性支持其在延迟分析的实验室测试中使用.
科学领域:
- 临床化学 临床化学
- 蛋白质分析 蛋白质分析
- 尿液诊断 尿液诊断
背景情况:
- 斯蛋白 (BJP) 是血细胞失分症的生物标志物.
- 准确量化BJP对于患者监测至关重要.
- 了解样本稳定性对于可靠的诊断测试至关重要.
研究的目的:
- 评估Bence Jones蛋白 (BJP) 在冷藏尿样中的短期稳定性.
- 为了确定BJP的度是否在5天的储存期内保持一致.
- 评估延迟分析对BJP测量的影响.
主要方法:
- 分析了20名患有血细胞失血症和BJP的患者的尿液样本.
- 样品在冷藏温度下保存长达5天.
- 与基线的百分比差异 (PD%) 计算在每日间隔,以量化变化.
主要成果:
- 印度人民党的M-spike百分比差异很大 (中位数为54%,范围为17-87%).
- 尿道蛋白质总水平也显示出显著的变化 (中位数为296 mg/L).
- 在5天内,没有观察到BJP集中的统计学意义上的趋势,PD%从-2%到+7%不等.
结论:
- 斯蛋白在冷藏尿液中表现出可接受的短期稳定性,长达5天.
- 这些发现支持在常规实验室环境中使用冷藏尿样进行BJP分析的可靠性.
- 在冷藏尿样中延迟分析BJP是可行的,而不会影响诊断的准确性.
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