活性氧物种在溶液和巨细胞中清理功能性富勒伦的容量
Rohin Biswas1, Brock J Manley2, Li Xiao2
1Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, 123 Bevier Rd, Piscataway, NJ 08854, United States.
概括
富勒烯衍生物通过清除活性氧物种 (ROS) 显示出抗氧化潜力. 它们的结构特征,总体大小和细胞吸收显著影响它们在生物系统中的有效性.
科学领域:
- 材料科学 材料科学 材料科学
- 生物化学 生化学
- 纳米技术 纳米技术
背景情况:
- 富勒烯衍生物因其激素清除和活性氧物种 (ROS) 灭能力而闻名.
- 它们作为生物系统中抗氧化剂的潜力正在调查中.
- 对于富勒衍生物的结构-活性关系缺乏明确的理解.
研究的目的:
- 为了研究结构特征与水溶性烯衍生物的激素清除效率之间的相关性.
- 在细胞模型中比较这些衍生物的抗氧化能力.
- 确定影响富勒烯衍生物作为抗氧化剂功效的关键参数.
主要方法:
- 合成八种代表性的水溶性烯衍生物.
- 在溶液中使用电子磁共振 (EPR) 评估激素清除特性.
- 对经过过氧化 (H2O2) 处理的RAW 264.7巨细胞中抗氧化能力的评估.
主要成果:
- 确定了影响激素清除和抗氧化作用的关键结构参数.
- 证明了功能组的数量和身份对C60的影响.
- 与RAW 264.7细胞中的抗氧化功效相关的总体大小和细胞吸收.
结论:
- 这项研究阐明了烯衍生物作为抗氧化剂的关键结构-活性关系.
- 功能组的修改,总体大小和细胞吸收是抗氧化剂活性的关键决定因素.
- 这些发现为设计更有效的基于富勒的抗氧化剂提供了洞察力.
相关概念视频
Peroxisomes
Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration—the process that converts energy from food into ATP. Peroxisomes carry out a variety of functions, primarily breaking down different substances, such as fatty acids.The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within peroxisomes...
Detergent Purification of Membrane Proteins
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
Lysosomes
Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...
Coagulation
Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...
Ion Exchange
Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or basic...
Protein Buffers in Blood Plasma and Cells
The human body utilizes protein buffer systems to maintain a stable pH. These systems capitalize on the dual role of amino acids, which can act as acids or bases by accepting or releasing hydrogen ions in response to pH changes. Protein buffer systems are particularly significant in the extracellular fluid (ECF) and intracellular fluid (ICF) of active cells, where structural and functional proteins provide substantial buffering capacity.
Certain amino acids can exist in a zwitterion state at a...
Certain amino acids can exist in a zwitterion state at a...


