Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

4.9K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
4.9K
RNA-seq03:21

RNA-seq

12.3K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.3K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

21.3K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
21.3K
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

7.1K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
7.1K
Protein Complex Assembly02:41

Protein Complex Assembly

17.0K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
17.0K
Protein Complex Assembly02:41

Protein Complex Assembly

2.6K
2.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Genetic and environmental determinants of multicellular-like phenotypes in fission yeast.

Genetics·2026
Same author

CXCR4 induces memory formation over exhaustion in CAR-T cells to achieve durable leukemia targeting.

Nature communications·2026
Same author

PomBase in 2026: expanding knowledge, modeling connections.

Genetics·2026
Same author

Mitochondrial Translation Inhibition Triggers an Rst2-Controlled Transcriptional Reprogramming of Carbon Metabolism in Stationary-Phase Cells of Fission Yeast.

Biomolecules·2025
Same author

The Insertion Domain of Mti2 Facilitates the Association of Mitochondrial Initiation Factors with Mitoribosomes in <i>Schizosaccharomyces pombe</i>.

Biomolecules·2025
Same author

Cell type-dependent directional transcription at enhancers.

NAR genomics and bioinformatics·2025
Same journal

Diel Activity Patterns of Bobcats and Domestic Cats in the Houston Metropolitan Area.

microPublication biology·2026
Same journal

Exploring the Effects of Antioxidants on αSynuclein-Induced Motor Deficits in <i>Drosophila</i> Larvae.

microPublication biology·2026
Same journal

Genetic disruption of <i>Capns1</i> impairs metastatic phenotypes in murine mammary carcinoma cells.

microPublication biology·2026
Same journal

The human GABA <sub>A</sub> receptor α4 subunit variant Ile114Asn linked to epilepsy impairs membrane expression with β3 and δ subunits.

microPublication biology·2026
Same journal

Estimating thermal death time curves in <i>Drosophila</i> <i>tripunctata</i> using infrared activity monitors.

microPublication biology·2026
Same journal

Revival of Mucin-Degrading Human-Derived Fecal Microbial Consortia After Cryopreservation.

microPublication biology·2026
查看所有相关文章

相关实验视频

Updated: Mar 10, 2026

Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group
07:49

Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group

Published on: August 16, 2017

7.5K

扩展Biopython,将多个序列对齐与相同引用结合到多个序列对齐中.

Cassia Bastress1, Michiel de Hoon2, Manuel Lera-Ramirez3

  • 1Faculty of Science, McGill University, Montréal, Canada.

microPublication biology
|March 9, 2026
PubMed
概括
此摘要是机器生成的。

这项研究引入了一种新的算法,将多个双向序列对齐 (PWA) 结合成一个单一的多重序列对齐 (MSA). 这种方法保留了对齐结构,有助于工作流程,例如使用测序痕迹进行循环等离子体验证.

更多相关视频

Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

9.2K
A Practical Guide to Phylogenetics for Nonexperts
12:00

A Practical Guide to Phylogenetics for Nonexperts

Published on: February 5, 2014

36.2K

相关实验视频

Last Updated: Mar 10, 2026

Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group
07:49

Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group

Published on: August 16, 2017

7.5K
Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

9.2K
A Practical Guide to Phylogenetics for Nonexperts
12:00

A Practical Guide to Phylogenetics for Nonexperts

Published on: February 5, 2014

36.2K

科学领域:

  • 生物信息学是一种生物信息学.
  • 计算生物学 计算生物学
  • 基因组数据分析 基因组数据分析

背景情况:

  • 配对对齐 (PWA) 对于与引用进行序列比较至关重要.
  • 目前的工具缺乏将单独对齐的PWA合并为单个多重序列对齐 (MSA) 的方法,同时保持结构完整性.
  • 特定的工作流程,例如用测序痕迹验证圆形质粒,需要将多个PWA组合到一个MSA中.

研究的目的:

  • 开发和实施一个算法,将多个PWA与一个共同的引用结合到一个单一的MSA中.
  • 确保在合并过程中保留原始对齐结构.
  • 为需要集成对齐可视化的工作流提供解决方案.

主要方法:

  • 开发了一种新的算法,将共享共同引用的PWA合并到一个MSA中.
  • 在 Biopython 的 Alignment 类中实现了算法作为一个类方法.
  • 算法旨在保持个别对齐结构的完整性.

主要成果:

  • 成功创建了一个算法,能够将多个PWA结合成一个统一的MSA.
  • 在BioPython中的实现有助于在生物信息学工作流程中的实际应用.
  • 该方法保留了原来的对齐结构,这对于准确的数据解释至关重要.

结论:

  • 开发的算法解决了序列对齐工具中的一个关键缺口.
  • 它可以从单个PWA创建全面的MSA,增强数据可视化和分析.
  • 这一进步支持复杂的基因组分析任务,包括循环遗传元素的验证.