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在小鼠中绘制肠道准细胞永久能力:在生理和刺激条件下的区域和细胞变化

Mathilde Miquel^1,2, Kadirey Verwaerde¹, Anissa Edir-Kibri³

¹Toxalim, Toulouse University, INRAE, ENVT, EI-Purpan Toulouse France.

FASEB bioAdvances

|March 9, 2026

概括

这项研究表明,肠道透性或"泄漏"发生在特定细胞周围,可以使用FM1-43FX (FM) 染料检测到. 慢性压力和脂质饮食被发现可以增加这种细胞透性.

科学领域:

胃肠病学胃肠病学
细胞生物学细胞生物学
生理学生理学生理学

背景情况:

肠道细胞透性对于恒温至关重要.
了解肠道透性的通路和调节对于诊断和治疗胃肠道疾病至关重要.

研究的目的:

用光染料来分析肠道细胞透率ex vivo.
研究慢性压力和脂质饮食对肠道透性的影响.
为了验证FM1-43FX (FM) 方法来检测增加的细胞透性.

主要方法:

在0°C下用光染料FM1-43FX (FM) 或TRITC-dextran 3 kDa氨酸可固定 (TD3L) 化小鼠肠道组织段.
使用共聚焦显微镜可视化染料透模式.
对健康小鼠,慢性压力 (葡萄糖皮质激素暴露) 的小鼠和食脂质饮食的小鼠进行了分析.

主要成果:

在健康小鼠中,FM和TD3L染料在小肠和结肠中的某些细胞类型 (小杯,肠内分泌细胞,,细胞) 周围显示出有限但特定的透.
这种在0°C的透模式表明了细胞的"泄漏"途径,与跨细胞内细胞分裂不同.
慢性压力增加了FITC-德克斯4kDa透性在体内和FM检测到的细胞透性体外在结肠中.
富含脂质的食增加了围绕阴肠细胞的ex vivo透性.

结论:

这项研究确定了在参与光感应的专门肠道细胞周围特定的细胞通路.
FM1-43FX (FM) 染料方法在检测和定位增高的细胞通透性时是有效的.
慢性压力和富含脂质的饮食等病理生理条件可以显著增加肠道细胞透性.

关键词:

慢性压力是一种慢性压力.肠内分泌细胞肠内分泌细胞玻璃杯细胞玻璃杯细胞我们的肠道是肠道.脂质的吸收脂质的吸收类似细胞的通透性.形细胞是形细胞的细胞.

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