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相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Total Internal Reflection Fluorescence Microscopy01:05

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
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超高分辨率成像使用光化石玻璃微球.

Haonan Zhuo1,2, Shengchuang Bai2, Zhouyi Yu2

  • 1Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Shenzhen China.

Nanophotonics (Berlin, Germany)
|March 9, 2026
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概括

高折射率的托化玻璃微球使超分辨率的光学纳米显微镜能够实现50nm分辨率. 这些微球为先进的纳米尺度成像和深层组织显微镜的潜在应用提供了一个多功能平台.

关键词:
托化石玻璃的使用方法微球微球是指一个微球.超高分辨率成像成像技术超显微镜对象 超显微镜对象

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科学领域:

  • 光学纳米显微镜的使用方法
  • 材料科学 材料科学 材料科学
  • 显微镜的使用方法

背景情况:

  • 传统的光学显微镜受 difraktion 的限制.
  • 微球透镜辅助的光学纳米学克服了衍射极限.
  • 高折射率的材料对于先进的纳米显微镜至关重要.

研究的目的:

  • 为了研究用于光学纳米镜的甲 (TBY) 玻璃微球.
  • 描述它们的光学特性和聚焦能力.
  • 为了展示它们在超高分辨率成像中的应用.

主要方法:

  • 使用浮带融技术制造TBY玻璃微球.
  • 微球属性的表征 (球性,表面,尺寸,折射率,透射率).
  • 射线追踪和电磁模拟用于近场聚焦分析.
  • 纳米样本的超高分辨率成像 (阳极氧化,格子).
  • 一个超显微镜目标 (UO) 模块的开发.

主要成果:

  • TBY微球具有出色的球状性,超光滑的表面,直径为10-200微米.
  • 折射率为1.9和可见透射率高达85%的可见透射率.
  • 展示了具有50nm分辨率和4.34×放大功率的超高分辨率成像.
  • 确定了图像平面选择和轴向对齐对图像质量的关键影响.
  • 开发了一种可重复使用的UO模块,与商业显微镜兼容.

结论:

  • TBY玻璃微球对于超高分辨率光学纳米学是有效的.
  • 优化的微球参数和对齐是高质量的成像的关键.
  • 开发的UO模块为纳米尺度成像提供了实际优势.
  • 甲玻璃显示出深层组织,多频段和激光微加工应用的潜力.