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使用Caco-2细胞模型系统评估结构多样化的植物化学品的透性相关行为和物理化学决定因素

Rixing Cong¹, Jin-Woo Kim¹, Jun-Su Park²

¹Department of Food Science and Biotechnology, Sejong University, 209, Neungdong-ro, Gwangjin-gu, 05006, Seoul, Republic of Korea. soonmishim@sejong.ac.kr.

Food & function

|March 9, 2026

概括

在Caco-2细胞中研究了162种植物化学物质,发现肠道透性因化学结构和脂性等特性而异. 了解这些关系对于预测植物化合物的生物可用性和疗效至关重要.

科学领域:

药理学和毒理学药理学和毒理学
营养科学营养科学
生物化学生物化学

背景情况:

植物化学肠道透性对于生物可用性和疗效至关重要.
不同的化学结构影响化合物与肠道相互作用的方式.
需要预测模型来理解这些相互作用.

研究的目的:

系统地研究162种不同的植物化学物质的肠道透性.
评估化学结构和物理化学特性对透性的影响.
使用Caco-2细胞模型探索结构-透性关系.

主要方法:

利用人类Caco-2细胞单层模型来评估肠道透性.
测量了屏障完整性的跨体电阻 (TEER).
确定了表面透系数 (Papp) 和外流比率 (ER).
与脂性 (X log P),TPSA,HBD和HBA等物理化学性质相关的透性.
使用主要组件分析 (PCA) 来可视化多变量关联.

主要成果:

植物化学物质对Caco-2细胞屏障完整性 (TEER) 产生了不同的影响.
表面透性 (Papp) 在化学类之间有很大的差异,其中类,类,黄类和类具有更高的透性.
金色化物,多糖和多具有较低的透性.
透性与脂友性正相关,与极地表面积和结能力负相关.
PCA表明,脂性和分子极性是透性的关键驱动因素,比单独的化学类更重要.

结论:

植物化学品的肠道透性是高度可变的,并受到物理化学性质的影响.
脂性和分子极性是通过肠道屏障被动扩散的重要预测因素.
卡科-2模型为各种植物化学品的结构-透性关系提供了宝贵的见解.
这些发现有助于预测植物化合物的生物可用性和疗效.

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