Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.3K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.3K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

7.2K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
7.2K
CRISPR and crRNAs02:53

CRISPR and crRNAs

19.4K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.4K
Antibiotic Selection00:57

Antibiotic Selection

61.7K
Overview
61.7K
CRISPR01:59

CRISPR

58.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Biocatalytic strategies for steroid synthesis: from side-chain remodeling to precision scaffold functionalization.

Chemical communications (Cambridge, England)·2026
Same author

An IDA peptide from Boea hygrometrica enhances desiccation tolerance but lacks activity in Arabidopsis.

Planta·2026
Same author

From islet to blood: macrophage remodeling signatures for diagnosis and risk stratification in type 1 diabetes.

Frontiers in immunology·2026
Same author

Mindful attention awareness, creative trait motivation, cognitive flexibility, and achievement goal orientation among medical students: a profile analysis and mediation analysis.

Frontiers in psychology·2026
Same author

Ivonescimab plus gemcitabine and cisplatin as first-line therapy for advanced biliary tract cancer: a multicenter, open-label phase 2 trial.

Journal of hepatology·2026
Same author

Severe bottleneck of ancient Homo populations: Insights from computational modeling and relevant fossil evidence.

Molecular biology and evolution·2026

相关实验视频

Updated: Mar 10, 2026

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

13.4K

在细菌中的多重准非特定基因组工程.

Runze Sun1, Ruixiang You1,2, Yiwen Zhou1,2

  • 1State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|March 9, 2026
PubMed
概括

一种新的基因组工程方法,MNGE,使不同细菌中代谢基因的稳定,多副本集成成为可能. 这种方法增强了生产有价值的化合物,如杀菌剂和抗生素.

关键词:
细菌 细菌 细菌是一种细菌.基因组工程是基因组工程.微生物药物 微生物药物多个目标的整合酶.合成生物学 合成生物学

更多相关视频

Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems
10:52

Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems

Published on: October 14, 2025

778
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

23.4K

相关实验视频

Last Updated: Mar 10, 2026

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

13.4K
Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems
10:52

Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems

Published on: October 14, 2025

778
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

23.4K

科学领域:

  • 微生物学 微生物学
  • 合成生物学 合成生物学
  • 代谢工程是代谢工程.

背景情况:

  • 基因组工程对于代谢工程和合成生物学至关重要.
  • 稳定的基因表达需要不同细菌的宿主独立整合技术.

研究的目的:

  • 开发一种可通用的基因组工程方法,用于在多种细菌中稳定,多副本的基因集成.
  • 为了证明这种方法在增强高价值化合物生产方面的有效性.

主要方法:

  • 开发了使用多重向整合酶 (MTI) 系统的多重向非特异性基因组工程 (MNGE).
  • 实现高度随机的多副本集成 (≥3副本),只需要核心TT二核酸.
  • 在阳性 (Streptomyces,Saccharopolyspora) 和阴性 (Burkholderia,Chromobacterium) 细菌中应用MNGE.

主要成果:

  • 成功地将真菌杀菌剂UK-2 BGC (41 kb) 和沙利诺米辛BGC (106 kb) 集成到Streptomyces albus中,提高了发酵水平.
  • 在 Burkholderia gladioli 中使用 MTI1 系统实现了 FR900359 BGC (66 kb) 的表达.
  • 证明了MNGE在各种细菌物种中的广泛适用性.

结论:

  • MNGE是一种多功能基因组工程工具,用于各种细菌.
  • 这种方法通过增强基因表达和集成,促进高价值化合物的高效生产.
  • MNGE为工业应用推进了下一代基因组工程.