阿斯卡里迪亚的分泌-分泌蛋白抑制肠道上皮细胞的扩散,并触发TLR4-介导的炎症
Xinyi Miao1, Zhe Cao1, Yuqing Chen1
1Anhui Province Key Laboratory of Animal Nutritional Regulation and Health, College of Animal Science, Anhui Science and Technology University, Fengyang, People's Republic of China.
概括
虫排泄分泌蛋白 (ESP) 通过阻碍修复和促进炎症来破坏肠上皮细胞 (IEC). 这种寄生虫通过这些复杂的机制破坏肠道屏障,从而影响鸟类的健康.
科学领域:
- 兽医寄生虫学 兽医寄生虫学
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 虫虫 (Ascaridia galli) 是一种常见的肠道寄生虫.
- A. galli分泌分泌蛋白 (ESP) 对肠上皮细胞 (IEC) 的作用及其机制尚不清楚.
研究的目的:
- 调查A. galli ESP在IECs上的局部化和影响.
- 阐明ESP引起的肠损伤背后的分子机制.
主要方法:
- 免疫组织化学被用来定位ESP.
- 通过LC-MS/MS确定了ESP组件.
- 实验室试验评估了ESP对IEC增殖,新陈代谢,信号转导和细胞循环的影响.
- 测量了促炎性细胞因子 (TNF-α,IL-6) 和氧化的产生.
- 分析了计费类受体4 (TLR4) 和IL-10表达水平.
主要成果:
- 在A. galli组织中发现了ESP,并与IEC结合.
- 已识别的ESP包括actin,热冲击蛋白,延长因子-1α和果糖二酸盐阿尔多酶.
- 通过破坏细胞代谢,信号转导和细胞循环,ESP抑制了IEC的扩散.
- ESP诱导了一种促炎反应 (增加TNF-α,IL-6,氧化),与TLR4升高和IL-10表达减少有关.
结论:
- A. galli ESP 通过抑制上皮质修复,损害了肠道屏障功能.
- 通过TLR4信号传递,ESP促进不适应性炎症,有助于鸟类虫病的发病.
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