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ATF6识别使B细胞前体急性淋巴细胞白血病细胞对多克索鲁比辛敏感

Samira Rahimi¹, Bahman Zarandi¹, Rima Manafi Shabestari¹

¹Department of Hematology and Blood Banking, School of Allied Medical Science, Iran University of Medical Science, Tehran, 1449614535, Iran.

Current medical science

|March 9, 2026

概括

激活转录因子6 (ATF6) 的抑制增强了多克索鲁比的作用.

科学领域:

分子生物学分子生物学
在瘤学瘤学.
细胞生物学细胞生物学

背景情况:

激活转录因子6 (ATF6) 是展开的蛋白质反应 (UPR) 和内质网膜压力调节的组成部分.
在各种癌症中注意到ATF6的过度表达,但其在B细胞前体急性淋巴细胞白血病 (BCP-ALL) 中的作用尚不清楚.

研究的目的:

研究ATF6在儿科BCP-ALL中的表达和功能意义.
评估ATF6抑制在BCP-ALL治疗中的治疗潜力.

主要方法:

分析了儿科ALL患者和健康对照组的骨髓样本.
用单独ATF6抑制剂 (Ceapin-A7) 和多克索鲁比辛治疗的BCP-ALL细胞系 (NALM-6,SUP-B15).
通过流细胞测量评估了亡;使用qRT-PCR和西方斑点分析了ATF6的基因和蛋白质表达,亡标记物和细胞循环调节剂.

主要成果:

与新诊断的患者相比,复发的ALL患者观察到更高的ATF6表达.
在BCP-ALL细胞系中,ATF6抑制与Ceapin-A7增强的多克索鲁比辛诱导的亡和G1细胞周期停止.
联合治疗调节了细胞亡和细胞循环调节途径;ATF6向基因显示复杂调节.

结论:

在BCP-ALL的发病过程中,ATF6发挥着重要作用.
将ATF6抑制与多克索鲁比结合起来是一个有前途的策略,可以增强BCP-ALL.的亡.

关键词:

激活转录因子6 (ATF6) 的作用.细胞灭亡 (apoptosis) 是一种死亡的过程.B细胞前体急性淋巴细胞白血病 (BCP-ALL)德克索鲁比辛是一种多克索鲁比辛.药物耐药性药物耐药性药物耐药性最小残留性疾病 (MRD) 是一种残留性疾病.不折叠的蛋白质反应反应

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