相关实验视频

Updated: Mar 11, 2026

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Published on: August 13, 2013

来自胃癌细胞的外体LINC01614驱动Treg分化

Nian He¹, Duji Ruan¹, Nianjie Zhang¹

¹Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Zunyi Medical University.

Journal of visualized experiments : JoVE

|March 9, 2026

概括

胃癌外体体中的长非编码RNA LINC01614促进调节性T细胞分化和M2巨细胞两极分化. 这种机制增强了瘤的扩散,并建议LINC01614作为免疫治疗的目标.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

胃癌 (GC) 的进展受到瘤免疫微环境的影响.
长非编码RNAs (lncRNAs) 与癌症有关,但它们在通过外体的GC免疫力中的作用尚不清楚.

研究的目的:

阐明LINC01614,一个lncRNA在通过外体调节GC免疫微环境中的机制.
研究LINC01614对T细胞分化和巨细胞两极分化的影响.

主要方法:

从与CD4 T细胞共同培养的GC细胞系中分离出外体,并进行了表征.
使用生物信息学数据库和qRT-PCR分析了LINC01614的表达.
功能性测试涉及透视病毒介导的LINC01614操纵,ELISA和西式涂抹,以评估免疫细胞反应和信号通路.

主要成果:

GC外体诱导CD4 T细胞分化成调节性T细胞 (Tregs).
在外体中LINC01614的过度表达促进了Treg IL-4分泌和M2巨细胞通过JAK-STAT3信号的两极分化,增强了TGF-β的释放.
这一过程显著促进了胃癌细胞的增殖.

结论:

通过外体,LINC01614驱动Treg分化和M2巨细胞极化,调节GC免疫微环境.
LINC01614为增强胃癌免疫疗法提供了一个潜在的治疗标.

更多相关视频

Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells

Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells

Published on: May 9, 2020

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Published on: April 16, 2012

相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...