产前营养调节Nellore牛后代的肝脏长非编码RNA调节元素
Guilherme Henrique Gebim Polizel1, Angela Cánovas2, Aline Silva Mello Cesar3
1Department of Animal Science, Faculty of Animal Science and Food Engineering - University of São Paulo, Av. Duque de Caxias Norte, 225, Pirassununga, SP, 13635-900, Brazil; Centre for Genetic Improvement of Livestock, Department of Animal Biosciences, University of Guelph, 50 Stone Road East, Guelph, Ontario, Canada.
The Journal of nutritional biochemistry
|March 9, 2026
概括
孕产妇的营养通过长非编码RNA (lncRNAs) 显著影响内罗尔牛的后代肝脏基因表达. 特定的饮食改变了能量代谢和与免疫功能相关的lncRNAs,为表观遗传编程提供了洞察力.
科学领域:
- 动物科学动物科学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 孕产妇的营养对胎儿发育和长期健康结果至关重要.
- 长非编码RNAs (lncRNAs) 是关键的表观遗传调节剂,在畜牧生产中扮演着新兴的角色.
研究的目的:
- 调查不同母亲饮食策略对内罗尔牛后代肝脏lncRNA表达的影响.
- 确定特定的lncRNA及其受产前营养影响的调节作用.
主要方法:
- 126只怀孕的内罗尔奶牛接受了不同的饮食治疗 (NP,PP,FP).
- 用RNA测序分析后代肝脏样本,以识别和量化lncRNAs.
- 生物信息工具 (FEELnc,CPC2,DESeq2) 用于lncRNA识别和差异表达分析.
- 监管网络分析 (部分相关性,监管影响因子) 确定了关键的 lncRNA 和丰富的生物过程.
主要成果:
- 确定了3,257个lncRNAs,其中17个被归类为差异表达的lncRNAs (DELs) (FDR <0.1).
- 与对照组 (NP) 相比,晚期蛋白质能量补充剂 (PP) 组显示出最显著的转录变化,发现了10个DEL.
- 特定的DEL,如TU7296,与能量代谢和免疫功能有关,而其他与脂肪酸运输和氧化蛋白质折叠有关.
结论:
- 怀孕期间母亲的营养通过lncRNA介导的表观遗传机制对肉牛后代的肝转录组产生持久的影响.
- 饮食中的蛋白质能量补充策略可以调节后代的代谢和免疫相关的基因表达特征.
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