所有你能吃的酵母:用AtSWEET7替换赫索斯载体减轻了葡萄糖抑制,使糖在可再生原料中同时利用
Nurzhan Kuanyshev1,2, Degaulle Dai1,2,3, Jungyeon Kim4
1Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
Biotechnology and bioengineering
|March 9, 2026
概括
现在,人工酵母通过用植物SWEET传送器取代本地传送器,共同发酵葡萄糖和其他糖类,如西洛斯. 这扩大了用于生物燃料生产的可再生原料的使用范围.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
背景情况:
- 酵母糖运输体表现出高葡萄糖偏好,限制了来自林氏细胞质生物质的各种糖的利用.
- 混合糖的高效发酵对于从可再生原料中生产具有成本效益的生物燃料至关重要.
研究的目的:
- 为了设计Saccharomyces cerevisiae同时同时消费多种糖,包括葡萄糖,西洛糖,曼诺糖和果糖.
- 克服本地赫索索转运器的局限性,并实现更广泛的基板利用.
主要方法:
- 在S. cerevisiae中用SWEET7 (AtSWEET7) 载体替换原生黑素载体 (HXT1-7).
- 在各种条件下在合成和工业介质上培育工程酵母菌株.
- 转录组和代谢组分析以了解代谢重编程.
主要成果:
- 改造的S. cerevisiae (NKSW7-1菌株) 显示葡萄糖偏好降低,同时对葡萄糖,曼诺斯,果糖和西洛斯进行共发酵.
- 连续培养证实了共同消费的表型,并缓解了葡萄糖抑制.
- 在NKSW7-1菌株中,也使用了氧化醇作为碳来源.
- 在AtSWEET7p表达时观察到中央碳代谢的全系统重编程.
结论:
- AtSWEET7运输器赋予S. cerevisiae广泛的糖运输能力,使其能够同时消费多种糖.
- 这种工程酵母平台具有显著的潜力,可以提高微生物细胞工厂在利用未充分利用的可再生原料方面的效率.
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