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Updated: Mar 11, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
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A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

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一个超保守的伪5'拼接站点通过调节TOR相关路径内的替代拼接来微调开发

Zhan Ding1,2,3, Zhuo-Ya Fang1,2,3, Hao Li1,2,3

  • 1Hubei Key Laboratory of Cell Homeostasis, Hubei Provincial Research Center for Basic Biological Sciences, State Key Laboratory of Virology and Biosafety, College of Life Sciences, Wuhan University, Wuhan, China.

Nature communications
|March 10, 2026
PubMed
概括

超保守的内基元素充当沉声器,通过与拼接机械相互作用来调节基因表达. 这种在物种中保持的机制影响卵巢发育,并响应营养信号通路.

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
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