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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Updated: Mar 11, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

生物发光探头用于多重复合RNA成像.

Erin B Fuller¹, Kyle H Cole², Lila P Halbers³

¹Department of Chemistry, University of California Irvine, Irvine, California 92617, United States.

Journal of the American Chemical Society

|March 10, 2026

概括

研究人员开发了新的生物发光RNA探针,称为RNA灯,用于多重RNA检测. 这些探测器可以在没有激发光的情况下对活细胞内的多个RNA点进行敏感,连续的可视化.

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Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

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Last Updated: Mar 11, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

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Published on: August 6, 2014

Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection

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科学领域:

分子生物学分子生物学
生物技术是生物技术.
细胞生物学细胞生物学

背景情况:

了解RNA生物学需要可视化RNA转录及其在生理环境中的实时相互作用的方法.
目前的技术在能够在生物系统中同时持续监测多个RNA物种的能力上是有限的.
生物发光探针提供了一个有前途的替代方案,因为它们的灵敏度和缺乏激发光的要求,使得非侵入性成像.

研究的目的:

开发和验证一种新的生物发光探针系统,用于在生物系统中检测多重RNA.
创建能够同时区分和报告多个RNA目标的直角RNA探针.
扩大RNA成像工具包,促进对RNA活体动态的研究.

主要方法:

设计和合成了一组结构化RNA标签和互补的光酶片段 (RNA灯) 的面板.
开发了三种正交标签-灯组合,利用已知的RNA结合蛋白用于特定的目标识别.
在体外优化探针性能,用于选择性转录检测,并在培养细胞中验证,用于多目标成像.

主要成果:

成功创建了RNA灯,只有当特定的RNA标签靠近时才能组装和发射光.
使用直角标签-灯组合,证明了对不同RNA目标的选择性检测.
在培养细胞中实现了具有良好的亚细胞分辨率的多目标RNA成像,展示了探针在生物环境中的有效性.

结论:

开发的RNA灯为多重RNA检测提供了一种新而敏感的方法.
这些探测器克服了现有技术的局限性,使生物系统中多种RNA物种的持续,非侵入性监测成为可能.
RNA灯系统显著扩大了RNA成像的能力,支持RNA生物学和动态的未来研究.